MESA+ Institute for Nanotechnology, University of Twente, AE Enschede, The Netherlands.
Biosens Bioelectron. 2011 Sep 15;27(1):77-81. doi: 10.1016/j.bios.2011.06.019. Epub 2011 Jun 23.
We introduce a sensing platform for specific detection of DNA based on the formation of gold nanoparticles dimers on a surface. The specific coupling of a second gold nanoparticle to a surface bound nanoparticle by DNA hybridization results in a red shift of the nanoparticle plasmon peak. This shift can be detected as a color change in the darkfield image of the gold nanoparticles. Parallel detection of hundreds of gold nanoparticles with a calibrated true color camera enabled us to detect specific binding of target DNA. This enables a limit of detection below 1.0×10(-14) M without the need for a spectrometer or a scanning stage.
我们介绍了一种基于金纳米粒子在表面上形成二聚体来进行特定 DNA 检测的传感平台。通过 DNA 杂交将第二个金纳米粒子特异性地连接到表面结合的纳米粒子上，导致纳米粒子等离子体峰发生红移。这种位移可以在金纳米粒子的暗场图像中检测到颜色变化。通过校准的真彩色相机对数百个金纳米粒子进行平行检测，我们能够检测到目标 DNA 的特异性结合。这使得在不需要光谱仪或扫描台的情况下，检测下限达到 1.0×10(-14) M 以下。
