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基于滚环扩增和缺口内切酶辅助的纳米颗粒扩增（NEANA）联用的超灵敏比色 DNA 检测

Ultrasensitive colorimetric DNA detection using a combination of rolling circle amplification and nicking endonuclease-assisted nanoparticle amplification (NEANA).

机构信息

Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore 117543.

出版信息

Small. 2012 Jun 25;8(12):1846-50. doi: 10.1002/smll.201200263. Epub 2012 Mar 28.

DOI:10.1002/smll.201200263
PMID:22461378
Abstract

A combination of rolling circle amplification and nicking endonuclease-assisted nanoparticle amplification (NEANA) is used for the rapid, colorimetric detection of DNA. The integration of rolling circle amplification into the NEANA approach allows for detection of oligonucleotides with arbitrary sequences at ultralow concentrations.

摘要

滚环扩增与缺口内切酶辅助纳米颗粒扩增（NEANA）的结合可用于快速比色法检测 DNA。将滚环扩增整合到 NEANA 方法中，可以在超低浓度下检测任意序列的寡核苷酸。

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