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后基因组时代的果蝇 RNAi 筛选。

Drosophila RNAi screening in a postgenomic world.

机构信息

Dynamical Cell Systems Laboratory, Division of Cancer Biology, The Institute of Cancer Research, London, UK.

出版信息

Brief Funct Genomics. 2011 Jul;10(4):197-205. doi: 10.1093/bfgp/elr015. Epub 2011 Jul 12.

DOI:10.1093/bfgp/elr015
PMID:21752787
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3144739/
Abstract

Drosophila melanogaster has a long history as a model organism with several unique features that make it an ideal research tool for the study of the relationship between genotype and phenotype. Importantly fundamental genetic principles as well as key human disease genes have been uncovered through the use of Drosophila. The contribution of the fruit fly to science and medicine continues in the postgenomic era as cell-based Drosophila RNAi screens are a cost-effective and scalable enabling technology that can be used to quantify the contribution of different genes to diverse cellular processes. Drosophila high-throughput screens can also be used as integral part of systems-level approaches to describe the architecture and dynamics of cellular networks.

摘要

黑腹果蝇作为一种模式生物，具有悠久的历史，其独特的特性使其成为研究基因型和表型关系的理想研究工具。重要的是，通过使用果蝇，发现了一些基本的遗传原理和关键的人类疾病基因。在后基因组时代，果蝇仍然为科学和医学做出贡献，基于细胞的果蝇 RNAi 筛选是一种具有成本效益和可扩展性的使能技术，可用于量化不同基因对不同细胞过程的贡献。果蝇高通量筛选也可以作为系统水平方法的一个组成部分，用于描述细胞网络的结构和动态。

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