Pediatric Research Institute, Ministry of Education Key Laboratory of Child Development and Disorders, Key Laboratory of Pediatrics in Chongqing, Chongqing International Science and Technology Cooperation Center for Child Development and Disorders, Children's Hospital of Chongqing Medical University, Chongqing, China.
J Clin Microbiol. 2011 Sep;49(9):3392-4. doi: 10.1128/JCM.00543-11. Epub 2011 Jul 13.
We compared a novel real-time genotyping and quantitative PCR (GQ-PCR) assay, direct sequence analysis, reverse hybridization, and multiplex PCR for genotyping hepatitis B virus (HBV) in 127 HBV-infected patients. We found that GQ-PCR had the highest concordance with sequence analysis and the highest detection rate for mixed genotype detecting.
我们比较了一种新型实时基因分型和定量 PCR(GQ-PCR)检测、直接测序分析、反向杂交和多重 PCR 用于检测 127 例乙型肝炎病毒(HBV)感染患者的基因分型。我们发现 GQ-PCR 与序列分析的一致性最高,且对混合基因型检测的检出率最高。