Langholz E, Nielsen O H, Ahnfelt-Rønne I, Elmgreen J
Department of Medical Gastroenterology C, University of Copenhagen, Herlev Hospital, Denmark.
Scand J Rheumatol. 1990;19(6):387-91. doi: 10.3109/03009749009097626.
Circulating human neutrophil granulocytes (PMNs) from patients with rheumatoid arthritis (RA) have earlier been described to possess an enhanced capacity for production of certain 5-lipoxygenase-derived metabolites of arachidonic acid (AA), 5-hydroxyeicosatetraenoic acid (5-HETE) and leukotriene B4 (LTB4). In the present investigation the endogenous AA metabolism of synovial fluid PMNs of RA patients was studied and compared with that of the corresponding circulating PMNs. Synovial fluid PMNs prelabelled with 14C-AA released significantly less radioactivity than circulating PMNs when stimulated with calcium ionophore. Furthermore, synovial fluid PMNs produced significantly smaller amounts of both 5-HETE and LTB4 than circulating PMNs from the same patients, whereas no such difference was observed in the LTB4 catabolites or the cyclo-oxygenase products. More information dealing with the complex way in which arachidonic acid is metabolized in diseased RA joints may provide future rational approaches in the treatment of this chronic inflammatory disease.
类风湿关节炎（RA）患者的循环人中性粒细胞（PMN）此前已被描述为具有增强的能力来产生某些花生四烯酸（AA）的5-脂氧合酶衍生代谢产物，即5-羟基二十碳四烯酸（5-HETE）和白三烯B4（LTB4）。在本研究中，对RA患者滑液PMN的内源性AA代谢进行了研究，并与相应的循环PMN进行了比较。当用钙离子载体刺激时，预先用14C-AA标记的滑液PMN释放的放射性明显少于循环PMN。此外，与同一患者的循环PMN相比，滑液PMN产生的5-HETE和LTB4的量明显更少，而在LTB4分解代谢产物或环氧化酶产物中未观察到这种差异。关于花生四烯酸在患病的RA关节中复杂代谢方式的更多信息可能为治疗这种慢性炎症性疾病提供未来合理的方法。
