Departamento de Imunologia, Microbiologia e Parasitologia Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Avenida Pará 1720, CEP 38400-902, Uberlândia, Minas Gerais, Brazil.
Parasitol Res. 2014 Jul;113(7):2569-75. doi: 10.1007/s00436-014-3908-7. Epub 2014 Apr 29.
Neurocysticercosis (NC) is one of the most important diseases caused by parasites affecting the central nervous system. We fractionated by ion-exchange chromatography using diethylaminoethyl (DEAE)-sepharose resin the total saline extract (S) from Taenia solium metacestodes and evaluated obtained fractions (DEAE S1 and DEAE S2) by enzyme-linked immunosorbent assay (ELISA, n = 123) and immunoblotting (IB, n = 22) to detect human NC in serum. Diagnostic parameters were established by ROC and TG ROC curves for ELISA tests. IB was qualitatively analyzed. S and DEAE S1 presented sensitivity of 87. 5% and DEAE S2 90%. The best specificity was observed for DEAE S2 (90.4%). In IB, using DEAE S2 samples from NC patients presented bands of 20-25, 43-45, 55-50, 60-66, 82, 89, and 140 kDa. The great diagnostic parameters reached by DEAE S2 suggest the potential applicability of this fraction in NC immunodiagnosis.
脑囊尾蚴病（NC）是由寄生虫引起的影响中枢神经系统的最重要疾病之一。我们使用 DEAE-琼脂糖树脂通过离子交换色谱法从猪带绦虫囊尾蚴的总盐提取物（S）中进行分级，并通过酶联免疫吸附试验（ELISA，n=123）和免疫印迹（IB，n=22）评估获得的级分（DEAE S1 和 DEAE S2），以检测血清中的人类 NC。通过 ROC 和 TG ROC 曲线为 ELISA 试验建立诊断参数。IB 进行定性分析。S 和 DEAE S1 的敏感性分别为 87.5%和 90%。DEAE S2 的最佳特异性为 90.4%。在 IB 中，使用来自 NC 患者的 DEAE S2 样本，出现了 20-25、43-45、55-50、60-66、82、89 和 140 kDa 的条带。DEAE S2 达到的巨大诊断参数表明该级分在 NC 免疫诊断中的潜在适用性。
