Infectious Diseases and Clinical Microbiology Unit, University Hospital Virgen Macarena, Avda Dr. Fedriani s/n., 41009, Seville, Spain.
Eur J Clin Microbiol Infect Dis. 2011 Sep;30(9):1045-7. doi: 10.1007/s10096-011-1168-3. Epub 2011 Jul 14.
The principal objective of this study was to assess whether chicken eggshells may be contaminated by ESBL-producing E. coli (ESBLC). Additional analyses were carried out to determine if ESBLEC could be detected in other foodstuffs such as cooked poultry or fresh vegetables. Seventy-two eggshells from different supermarkets and stores as well as 32 salads, 30 samples of cooked poultry and six samples of chicken-based pet food samples were analysed. Characterization of ESBL was performed by PCR and sequencing. Antimicrobial resistance was determined by disk diffusion method. Phylogenetic group was assigned by multiplex-PCR. No ESBLEC was isolated from chicken eggshells, cooked chicken and pet food. One SHV-12-producing E. coli was isolated from a salad sample. This is the first study to analyse chicken eggs in an area where there is a high prevalence of ESBLEC in retail chicken meat.
本研究的主要目的是评估鸡蛋壳是否可能被产 ESBL 的大肠杆菌（ESBLC）污染。还进行了其他分析，以确定是否可以在其他食品（如熟食家禽或新鲜蔬菜）中检测到 ESBLEC。分析了来自不同超市和商店的 72 个蛋壳，以及 32 份沙拉、30 份熟食家禽和 6 份以鸡肉为基础的宠物食品样本。通过 PCR 和测序进行 ESBL 特征分析。通过圆盘扩散法测定抗药性。通过多重 PCR 确定了进化群。未从鸡蛋壳、熟食鸡肉和宠物食品中分离出 ESBLEC。从一份沙拉样本中分离出一株产 SHV-12 的大肠杆菌。这是首次在零售鸡肉中 ESBLEC 流行率较高的地区分析鸡蛋的研究。
