Institut Pasteur in Cambodia, Réseau International des Instituts Pasteur, 5 Monivong Boulevard, PO Box 983, Phnom Penh, Cambodia.
Infect Genet Evol. 2013 Apr;15:59-68. doi: 10.1016/j.meegid.2011.06.019. Epub 2011 Jul 2.
In Cambodia, dengue virus (DENV) was first isolated in 1963 and has become endemic with peak epidemic during raining season. Since 2000, the Dengue National Control Program has reported from 10,000 to 40,000 cases per year with fatality rates ranging from 0.7 to 1.7. All four dengue serotypes are found circulating in Cambodia with alternative predominance of serotypes DENV-2 and DENV-3. The DENV-1 represents from 5% to 20% of all circulating viruses, depending upon the year. In this work, 79 clinical strains of DENV-1 were isolated between 2000 and 2009 and their genome fully sequenced. Four distinct lineages with different dynamics were identified. The main evolutionary drive was negative selective pressure but each lineage was characterized by the presence of specific mutations acquired through evolution. Coexistence, extinction and replacement of lineages occurred over the 10-year period. Lineages 1, 2 and 3 were all detected since 2000-2002 and disappeared in 2003, 2004-2005 and 2007, respectively. Lineages 1 and 2 displayed different dynamics. Lineage 1 was very diverse whereas lineage 2 was very homogeneous. Lineage 4 which derived from lineage 3 in 2003 remained the only one at the end of the sampling period in 2008-2009 owing to a selective sweep. The lineages dynamic of DENV-1 viruses and consequences for molecular epidemiology are discussed.
在柬埔寨，登革热病毒（DENV）于 1963 年首次分离，并在雨季出现流行高峰，现已成为地方病。自 2000 年以来，登革热国家控制计划每年报告 10,000 至 40,000 例病例，死亡率在 0.7%至 1.7%之间。柬埔寨流行的所有四种登革热血清型都有交替优势，血清型 DENV-2 和 DENV-3 占主导地位。登革热 1 型代表所有循环病毒的 5%至 20%，具体取决于年份。在这项工作中，2000 年至 2009 年间分离出了 79 株登革热 1 型临床株，并对其基因组进行了全序列测序。确定了具有不同动态的四个不同谱系。主要进化驱动力是负选择压力，但每个谱系的特征是通过进化获得的特定突变的存在。在 10 年期间，谱系共存、灭绝和替代同时发生。谱系 1、2 和 3 均于 2000-2002 年检测到，并于 2003 年、2004-2005 年和 2007 年分别消失。谱系 1 和 2 显示出不同的动态。谱系 1 非常多样化，而谱系 2 非常同质。谱系 4 于 2003 年源自谱系 3，由于选择压力，它在 2008-2009 年的采样期末成为唯一的谱系。讨论了登革热 1 型病毒的谱系动态及其对分子流行病学的影响。
