巴西和巴拉圭分离的登革热病毒 3 型的系统进化关系和全球进化离散动力学。
Phylogenetic relationship of dengue virus type 3 isolated in Brazil and Paraguay and global evolutionary divergence dynamics.
机构信息
Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Universidade de São Paulo, Av, do Café s/n., 14040-903, Ribeirão Preto, São Paulo, Brazil.
出版信息
Virol J. 2012 Jun 20;9:124. doi: 10.1186/1743-422X-9-124.
BACKGROUND
Dengue is the most important mosquito-borne viral disease worldwide. Dengue virus comprises four antigenically related viruses named dengue virus type 1 to 4 (DENV1-4). DENV-3 was re-introduced into the Americas in 1994 causing outbreaks in Nicaragua and Panama. DENV-3 was introduced in Brazil in 2000 and then spread to most of the Brazilian States, reaching the neighboring country, Paraguay in 2002. In this study, we have analyzed the phylogenetic relationship of DENV-3 isolated in Brazil and Paraguay with viruses isolated worldwide. We have also analyzed the evolutionary divergence dynamics of DENV-3 viruses.
RESULTS
The entire open reading frame (ORF) of thirteen DENV-3 isolated in Brazil (n = 9) and Paraguay (n = 4) were sequenced for phylogenetic analysis. DENV-3 grouped into three main genotypes (I, II and III). Several internal clades were found within each genotype that we called lineage and sub-lineage. Viruses included in this study belong to genotype III and grouped together with viruses isolated in the Americas within the lineage III. The Brazilian viruses were further segregated into two different sub-lineage, A and B, and the Paraguayan into the sub-lineage B. All three genotypes showed internal grouping. The nucleotide divergence was in average 6.7% for genotypes, 2.7% for lineages and 1.5% for sub-lineages. Phylogenetic trees constructed with any of the protein gene sequences showed the same segregation of the DENV-3 in three genotypes.
CONCLUSION
Our results showed that two groups of DENV-3 genotypes III circulated in Brazil during 2002-2009, suggesting different events of introduction of the virus through different regions of the country. In Paraguay, only one group DENV-3 genotype III is circulating that is very closely related to the Brazilian viruses of sub-lineage B. Different degree of grouping can be observed for DENV-3 and each group showed a characteristic evolutionary divergence. Finally, we have observed that any protein gene sequence can be used to identify the virus genotype.
背景
登革热是全球最重要的蚊媒病毒病。登革病毒由 4 种具有不同抗原性的病毒组成，分别命名为登革病毒 1 型至 4 型（DENV1-4）。DENV-3 于 1994 年重新引入美洲，导致尼加拉瓜和巴拿马暴发疫情。DENV-3 于 2000 年传入巴西，随后传播到巴西的大多数州，并于 2002 年传播到邻国巴拉圭。在本研究中，我们分析了巴西和巴拉圭分离的 DENV-3 与全球分离的病毒之间的系统进化关系，并分析了 DENV-3 病毒的进化分歧动态。
结果
对巴西（n=9）和巴拉圭（n=4）分离的 13 株 DENV-3 的整个开放阅读框（ORF）进行了测序，用于系统进化分析。DENV-3 分为 3 个主要基因型（I、II 和 III）。在每个基因型内发现了几个内部分支，我们将其称为谱系和亚谱系。本研究中包含的病毒属于基因型 III，与美洲分离的病毒一起属于 III 谱系。巴西病毒进一步分为两个不同的亚谱系 A 和 B，巴拉圭病毒分为 B 亚谱系。所有 3 个基因型均显示内部分组。基因型的核苷酸差异平均为 6.7%，谱系为 2.7%，亚谱系为 1.5%。使用任何蛋白基因序列构建的系统进化树均显示出 3 种基因型 DENV-3 的相同分组。
结论
本研究结果表明，2002-2009 年期间，巴西有两组基因型 III 的 DENV-3 循环，提示病毒通过该国不同地区传入。在巴拉圭，仅循环一组非常密切相关的 DENV-3 基因型 III 的 B 亚谱系病毒。可以观察到 DENV-3 的不同程度分组，每组均显示出特征性的进化分歧。最后，我们发现可以使用任何蛋白基因序列来识别病毒基因型。
Zotero 插件