Department of Biomedical Sciences, G. d'Annunzio University, Chieti-Pescara, Italy.
J Proteomics. 2011 Nov 18;74(12):2826-36. doi: 10.1016/j.jprot.2011.06.023. Epub 2011 Jul 4.
Multiple Sclerosis (MS) is a neurodegenerative autoimmune demyelinating disease affecting young adults. The aetiology still remains a mystery and diagnosis is impaired by the lack of defined molecular markers. Autoimmune response remains the main topic under investigation and recent studies suggest additional non-proteic mediators of brain inflammation such as lipids. We carried out an LC-MS based lipidomics approach to highlight serum lipids profiling in MS. Method was optimised and applied in a preliminary clinical cross-sectional investigation of MS patients vs Healthy Controls (HC) and patients with Other Neurological Diseases (OND). Ten significant metabolites were highlighted and tentatively identified by accurate mass and MS/MS experiments. Our most relevant data show altered level of lyso-glycerophosphatidylcholine (lysoPC) and glycerophosphatidylcholine (PC) species. Total lysoPC/PC ratio showed significant decrease in pathological groups (MS, OND) and, in addition, MS subjects had a relevant decrease of this ratio also in respect to OND. These findings suggest that there may be an altered phospholipid metabolism in MS that can be evaluated in serum. Some of these features are distinctive and may be considered specific for MS. Our lipidomics data show, for the first time, evidence in serum of a relationship between LysoPC/PC ratio and MS.
多发性硬化症（MS）是一种影响年轻人的神经退行性自身免疫性脱髓鞘疾病。其病因仍然是个谜，并且由于缺乏明确的分子标志物，诊断受到了影响。自身免疫反应仍然是研究的主要课题，最近的研究表明，脑炎症的其他非蛋白介质，如脂质，也有参与。我们进行了基于 LC-MS 的脂质组学方法，以突出多发性硬化症患者血清脂质谱的特征。对该方法进行了优化，并应用于多发性硬化症患者与健康对照（HC）和其他神经疾病（OND）患者的初步临床横断面研究。通过精确质量和 MS/MS 实验，突出了 10 种有意义的代谢物，并对其进行了初步鉴定。我们最相关的数据显示，溶血甘油磷脂酰胆碱（lysoPC）和甘油磷脂酰胆碱（PC）种类的水平发生了改变。在病理组（MS、OND）中，总 lysoPC/PC 比值显著降低，此外，MS 患者与 OND 患者相比，该比值也有明显降低。这些发现表明，MS 中可能存在改变的磷脂代谢，可以在血清中进行评估。其中一些特征具有特异性，可能被认为是 MS 的特征。我们的脂质组学数据首次表明，血清中 LysoPC/PC 比值与 MS 之间存在关联。
