Electrophoresis. 2013 Oct;34(19):2848-56.
In this study, an ultra fast LC/IT-TOF MS (UFLC/IT-TOF MS)-based serum lipidomics method was employed to characterize the serum lipid profile of patients with chronic hepatitis B, cirrhosis, and hepatocellular carcinoma (HCC). After data collection and processing, 96 lipids including lysophosphatidylcholines, phosphatidylcholines, sphingomyelins, triacylglycerides, and cholesterol esters were identified and used for subsequent data analysis. Partial least squares-discriminant analysis revealed that patients with liver diseases had distinctly different serum lipid profile from that of healthy controls; while cirrhosis and HCC patients had a similar serum lipid profile, but different from that of hepatitis patients. The ANOVA analysis found 75 of the 96 identified lipids to be abnormally regulated, among which most of these lipids were downregulated in cirrhosis and HCC patients compared with those of healthy controls and hepatitis patients, while hepatitis patients induced several lipids downregulated and others upregulated compared with those of healthy controls, indicating the aberrant lipid metabolism in patients with liver diseases. This work demonstrated the utility of UFLC/IT-TOF MS-based serum lipidomics as a powerful tool to investigate the lipid metabolism of liver diseases.
在这项研究中，我们采用基于超快速 LC/IT-TOF MS（UFLC/IT-TOF MS）的血清脂质组学方法来描绘慢性乙型肝炎、肝硬化和肝细胞癌（HCC）患者的血清脂质图谱。在数据收集和处理后，共鉴定出 96 种脂质，包括溶血磷脂酰胆碱、磷脂酰胆碱、鞘磷脂、三酰基甘油和胆固醇酯，并用于后续的数据分析。偏最小二乘判别分析显示，肝病患者的血清脂质图谱与健康对照组明显不同；而肝硬化和 HCC 患者的血清脂质图谱相似，但与肝炎患者不同。方差分析发现 96 种鉴定出的脂质中有 75 种存在异常调节，其中大多数在肝硬化和 HCC 患者中下调，而与健康对照组和肝炎患者相比，肝炎患者诱导了一些脂质下调和其他脂质上调，表明肝病患者的脂质代谢异常。这项工作证明了基于 UFLC/IT-TOF MS 的血清脂质组学作为研究肝脏疾病脂质代谢的有力工具的实用性。
