Serological and Molecular Detection of Species in Stray Dogs and Investigation of DNA by PCR in Turkey.

BACKGROUND

and are the most common species of filarial nematodes described in the dogs. A single-step multiplex PCR was applied to detect and differentiate simultaneously and unequivocally and on DNA extracted from canine peripheral blood and besides to detect the seroprevalance of by ELISA in Elazig Province, Turkey. A PCR detection of the , which plays an important role in biology and contributes to the inflammatory pathology of the heartworm, was also applied for the first time in Turkey.

METHODS

A total of 161 whole blood and sera samples were collected from stray dogs and stored at -20 °C until used. After DNA extraction, all samples were processed with primers by multiplex-PCR and primers by conventional PCR besides ELISA for serology. The amplification was performed using a set of primers designed on a portion of the small subunit ribosomal RNA gene of the mitochondrion (12S rDNA).

RESULTS

Three of the examined dogs (1.8%) were found to be infected with only , one (0.6%) with and three (1.8%) with both parasites. Besides, 10 out of 161 dogs (6.2%) were found infected with sp. Finaly, the seroprevalence of dirofilariosis in the examined dogs was found to be 3.7% (6/161).

CONCLUSION

Although dirofilariosis is not a serious problem in the region, the stray dogs still continue to be a source of infection.