Milanowski J, Dutkiewicz J
Clinic of Lung Diseases, University School of Medicine, Lublin, Poland.
Allergol Immunopathol (Madr). 1990 Jul-Aug;18(4):211-5.
The gaseous phase cultures of alveolar macrophages (AMs) of guinea pig were exposed to the saline extracts of the dust-borne bacteria Micropolyspora faeni (syn. Faenia rectivirgula) and Erwinia herbicola (syn. Enterobacter agglomerans) which have been added at the concentration of 1 microgram/ml to culture medium with or without complement. The effects of exposure on superoxide anion (O2-) production by AMs were assessed by the lucigenin-dependent chemiluminescence method. Both extracts caused significant (p less than 0.01) increase in O2- generation by AMs, as assessed by the 161-254% enhancement of chemiluminescence release comparing to control values. The presence of complement augmented the production of O2-, which reached a peak at 3 hrs after initial exposure. The possible significance of the generation of oxygen radicals in pathogenesis of the diseases due to exposure to agricultural dusts loaded with bacterial antigens is discussed.
将豚鼠肺泡巨噬细胞（AMs）的气相培养物暴露于尘埃携带细菌微小多孢菌（同义词：直链嗜热放线菌）和草生欧文氏菌（同义词：成团肠杆菌）的盐水提取物中，这些提取物已以1微克/毫升的浓度添加到有或没有补体的培养基中。通过光泽精依赖性化学发光法评估暴露对AMs产生超氧阴离子（O2-）的影响。与对照值相比，通过化学发光释放增强161-254%评估，两种提取物均导致AMs产生的O2-显著（p<0.01）增加。补体的存在增强了O2-的产生，在初次暴露后3小时达到峰值。讨论了由于暴露于负载细菌抗原的农业粉尘而产生的氧自由基在疾病发病机制中的可能意义。
