KS-215, Advanced Centre for Treatment Research Education and Cancer, Tata Memorial Centre, Kharghar Node, Navi Mumbai, India.
PLoS One. 2011;6(7):e21975. doi: 10.1371/journal.pone.0021975. Epub 2011 Jul 7.
This report describes a technique for the generation of transgenic mice by in vivo manipulation of spermatogonial stem cells with a high rate of success. Spermatogonial stem cells (SSCs) in pre-pubescent animals were infected in vivo with recombinant lentiviruses expressing EGFP-f and mated with normal females. All male pre-founder mice produced transgenic pups with an overall success rate of over 60%. The transgene was heritable and the pre-founder mice could be used in multiple mating experiments. This technology could be used to perform overexpression/knockdown screens in vivo using bar-coded lentiviruses, thus permitting the design of genetic screens in the mouse. Further, this technology could be adapted to other laboratory animals resulting in the generation of model systems that closely approximate human development and disease.
本报告描述了一种通过体内操作精原干细胞高效产生转基因小鼠的技术。将表达 EGFP-f 的重组慢病毒体内感染青春期前动物的精原干细胞，并与正常雌性小鼠交配。所有雄性前导鼠都产生了转基因幼鼠，整体成功率超过 60%。转基因是可遗传的，前导鼠可用于多次交配实验。该技术可用于使用条形码慢病毒在体内进行过表达/敲低筛选，从而可以在小鼠中设计遗传筛选。此外，该技术可以适应其他实验动物，从而产生更接近人类发育和疾病的模型系统。
