以貉藻（Drosera aliciae）的体外培养物作为细胞毒性萘醌：拉蒙塔西酮的来源。

In vitro cultures of Drosera aliciae as a source of a cytotoxic naphthoquinone: ramentaceone.

机构信息

Department of Biotechnology, Intercollegiate Faculty of Biotechnology, University of Gdansk & Medical University of Gdansk, Gdansk, Poland.

出版信息

Biotechnol Lett. 2011 Nov;33(11):2309-16. doi: 10.1007/s10529-011-0700-y. Epub 2011 Jul 15.

DOI:10.1007/s10529-011-0700-y

PMID:21761256

Abstract

A protocol for the in vitro propagation of Drosera aliciae to increase the yield of the naphthoquinone, ramentaceone, was developed. The highest micropropagation coefficient was obtained using half-strength Murashige-Skoog medium supplemented with 0.4 μM 6-benzyladenine (BA). The genetic fidelity and stability of the regenerated plants was confirmed with RAPD markers. The activity of the isolated ramentaceone was determined against four human tumor cell lines: U937, HeLa, MCF-7, HCT-116 with the highest cytotoxic activity towards the leukemic U937 cell line with an IC(50) value of 3.2 μM.

摘要

为提高萘醌化合物岩白菜素的产量，我们制定了一种用于离体繁殖茅膏菜的方案。结果表明，在附加 0.4μM 6-苄基腺嘌呤的半强度 Murashige-Skoog 培养基中，微繁殖系数最高。利用 RAPD 标记物确认了再生植株的遗传保真度和稳定性。对分离得到的岩白菜素进行了活性测试，结果表明，它对四种人肿瘤细胞系（U937、HeLa、MCF-7 和 HCT-116）具有活性，对白血病 U937 细胞系的细胞毒性活性最高，IC(50)值为 3.2μM。