用于分析福尔马林固定石蜡包埋组织中基因表达的激光捕获显微切割技术。

Laser capture microdissection for analysis of gene expression in formalin-fixed paraffin-embedded tissue.

作者信息

Jiang Ru, Scott Rona S, Hutt-Fletcher Lindsey M

机构信息

Department of Microbiology and Immunology, Center for Molecular and Tumor Virology and Feist-Weiller Cancer Center, Louisiana State University Health Sciences Center, Shreveport, LA, USA.

出版信息

Methods Mol Biol. 2011;755:77-84. doi: 10.1007/978-1-61779-163-5_6.

DOI:10.1007/978-1-61779-163-5_6

PMID:21761295

Abstract

A combination of laser capture microdissection and reverse transcriptase real-time quantitative PCR -provides a powerful tool for the analysis of relative gene expression in archived tissue specimens. This chapter describes standard methodologies that can be used to determine the relative levels of gene expression in individual cells captured from formalin-fixed paraffin-embedded tissues.

摘要

激光捕获显微切割与逆转录实时定量PCR相结合，为分析存档组织标本中的相对基因表达提供了一个强大的工具。本章介绍了可用于确定从福尔马林固定石蜡包埋组织中捕获的单个细胞中基因表达相对水平的标准方法。