使用来自显微切割免疫染色组织的RNA进行微小RNA分析。

MicroRNA profiling using RNA from microdissected immunostained tissue.

作者信息

Bockmeyer Clemens L, Jonigk Danny, Kreipe Hans, Lehmann Ulrich

机构信息

Institute of Pathology, Medizinische Hochschule Hannover, Hannover, Germany.

出版信息

Methods Mol Biol. 2011;755:85-94. doi: 10.1007/978-1-61779-163-5_7.

DOI:10.1007/978-1-61779-163-5_7

PMID:21761296

Abstract

mRNA expression profiling has been used to define molecular subtypes of human breast cancer. Also microRNAs have been investigated in these breast cancer subtypes. However, little is known regarding the microRNA signature of healthy luminal and basal breast epithelial cells. Therefore, a method is described to isolate immunostained luminal and basal breast epithelial cells in formalin-fixed paraffin-embedded tissues by laser microdissection. Employing this new methodological approach, we could identify distinct microRNA profiles of luminal and basal breast epithelial cells by real-time PCR-based profiling.

摘要

mRNA表达谱已被用于定义人类乳腺癌的分子亚型。同时，也对这些乳腺癌亚型中的微小RNA进行了研究。然而，关于健康的管腔型和基底型乳腺上皮细胞的微小RNA特征却知之甚少。因此，本文描述了一种通过激光显微切割从福尔马林固定石蜡包埋组织中分离免疫染色的管腔型和基底型乳腺上皮细胞的方法。采用这种新的方法，我们能够通过基于实时PCR的分析确定管腔型和基底型乳腺上皮细胞不同的微小RNA谱。