Department of Biochemistry and Cell Biology, University of Rzeszów, Poland.
Yeast. 2011 Aug;28(8):595-609. doi: 10.1002/yea.1889. Epub 2011 Jul 14.
Sodium hypochlorite and sodium chlorite are commonly used as disinfectants, and understanding the mechanisms of microbial resistance to these compounds is of considerable importance. In this study, the role of oxidative stress and antioxidant enzymes in the sensitivity of the yeast Saccharomyces cerevisiae to hypochlorite and chlorite was studied. Yeast mutants lacking Cu-Zn superoxide dismutase, but not mutants deficient in cytoplasmic and peroxisomal catalase, were hypersensitive to the action of both hypochlorite and chlorite. Both compounds depleted cellular glutathione, induced the production of reactive oxygen species and decreased the viability of the cells. The toxicity of hypochlorite and chlorite was abolished by hypoxic and anoxic conditions and ameliorated by thiol antioxidants and ascorbate. The results demonstrated that the action of hypochlorite and chlorite involves the formation of superoxide and peroxide and that SOD1 is protective, probably by limiting the formation of hydroxyl radicals and damage to proteins.
次氯酸钠和亚氯酸钠常用于消毒剂，了解微生物对这些化合物产生抗性的机制具有重要意义。在这项研究中，研究了氧化应激和抗氧化酶在酵母酿酒酵母对次氯酸盐和亚氯酸盐敏感性中的作用。缺乏 Cu-Zn 超氧化物歧化酶的酵母突变体对次氯酸盐和亚氯酸盐的作用敏感，但缺乏细胞质和过氧化物酶体过氧化氢酶的突变体则不敏感。这两种化合物都会耗尽细胞中的谷胱甘肽，诱导活性氧的产生，并降低细胞的活力。缺氧和无氧条件可消除次氯酸盐和亚氯酸盐的毒性，并可通过巯基抗氧化剂和抗坏血酸加以改善。结果表明，次氯酸盐和亚氯酸盐的作用涉及超氧化物和过氧化物的形成，而 SOD1 具有保护作用，可能是通过限制羟基自由基的形成和蛋白质损伤。
