Jaruga E, Lapshina E A, Biliński T, Płonka A, Bartosz G
Department of Molecular Biophysics, University of Lódź.
Biochem Mol Biol Int. 1995 Oct;37(3):467-73.
Radiation sensitivity of the yeast (Saccharomyces cerevisiae) strains defective in catalase and superoxide dismutase, and of decreased level of glutathione was compared. Small differences in the radiation sensitivity pointed to the highest sensitivity of the wild-type strain as compared with the antioxidant-defective mutants. Heat shock increases the radiation resistance to a different extent, the response being highest in the wild-type strain and lowest in the glutathione-deficient strain. Catalase induction by heat shock follows the same pattern, significant activity of this enzyme being detectable in the glutathione-deficient mutant under normal growth conditions. These results suggest that compensatory induction of elements of the antioxidant defence system may occur in yeast mutants deficient in antioxidants under normal growth conditions.
对过氧化氢酶和超氧化物歧化酶有缺陷以及谷胱甘肽水平降低的酵母（酿酒酵母）菌株的辐射敏感性进行了比较。辐射敏感性的微小差异表明，与抗氧化缺陷型突变体相比，野生型菌株的敏感性最高。热休克在不同程度上增加了辐射抗性，野生型菌株的反应最高，而谷胱甘肽缺乏型菌株的反应最低。热休克诱导过氧化氢酶遵循相同的模式，在正常生长条件下，在谷胱甘肽缺乏型突变体中可检测到该酶的显著活性。这些结果表明，在正常生长条件下，抗氧化防御系统元件的补偿性诱导可能发生在抗氧化剂缺乏的酵母突变体中。
