兔 VX2 肝肿瘤射频消融的四维经导管动脉内灌注 MRI 监测。

Four-dimensional transcatheter intraarterial perfusion MRI monitoring of radiofrequency ablation of rabbit VX2 liver tumors.

机构信息

Department of Radiology, Northwestern University, Chicago, Illinois, USA.

出版信息

J Magn Reson Imaging. 2011 Sep;34(3):563-9. doi: 10.1002/jmri.22644. Epub 2011 Jul 14.

DOI:10.1002/jmri.22644

PMID:21761464

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3527903/

Abstract

PURPOSE

To investigate the hypothesis that four-dimensional (4D) transcatheter intraarterial perfusion (TRIP) magnetic resonance imaging (MRI) can quantify immediate perfusion changes after radiofrequency (RF) ablation in rabbit VX2 liver tumors.

MATERIALS AND METHODS

Nine New Zealand White rabbits were used to surgically implant VX2 liver tumors. During ultrasound-guided RF ablation, tumors received either a true or sham ablation. After selective catheterization of the left hepatic artery under x-ray fluoroscopy, we acquired pre- and post-RF ablation 4D TRIP MR images using 3 mL of 2.5% intraarterial gadopentetate dimeglumine. Two regions-of-interest were drawn upon each tumor to generate signal-intensity time curves. Area under the curve (AUC) was calculated to provide semiquantitative perfusion measurements that were compared using a paired t-test (α = 0.05). Ablated tissue was visually confirmed on pathology using Evans blue dye.

RESULTS

Mean AUC perfusion of VX2 tumors for the true ablation group decreased by 92.0% (95% confidence interval [CI]: 83.3%-100%), from 1913 (95% CI: 1557, 2269) before RF ablation to 76.6 (95% CI: 18.4, 134.8) after RF ablation (a.u., P < 0.001). Sham-ablated tumors demonstrated no significant perfusion changes.

CONCLUSION

4D TRIP MRI can quantify liver tumor perfusion reductions in VX2 rabbits after RF ablation. This MRI technique can potentially be used to improve tumor response assessment at the time of RF ablation.

摘要

目的

研究假设即四维（4D）经导管动脉内灌注（TRIP）磁共振成像（MRI）是否可以定量分析兔 VX2 肝肿瘤射频（RF）消融后即刻灌注变化。

材料与方法

使用 9 只新西兰白兔进行手术植入 VX2 肝肿瘤。在超声引导下进行 RF 消融时，肿瘤接受真实消融或假消融。在 X 射线透视下选择性地对左肝动脉进行导管插入后，我们使用 3 毫升 2.5%的动脉内钆喷替酸二甲胺，在 RF 消融前后获取 4D TRIP MRI 图像。在每个肿瘤上绘制 2 个感兴趣区以生成信号强度时间曲线。计算曲线下面积（AUC）以提供半定量灌注测量值，并使用配对 t 检验进行比较（α=0.05）。使用 Evans 蓝染料在病理学上对消融组织进行视觉确认。

结果

真实消融组的 VX2 肿瘤平均 AUC 灌注下降了 92.0%（95%置信区间 [CI]：83.3%-100%），从 RF 消融前的 1913（95% CI：1557，2269）降至 RF 消融后的 76.6（95% CI：18.4，134.8）（a.u.，P <0.001）。假消融的肿瘤没有显示出明显的灌注变化。