Zhang Zhiyuan, Xu Meiling, Zhang Jian
Department of Otolaryngology Head and Neck Surgery, the First Affiliated Hospital of Nanchang University, Nanchang, 330006, China.
Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2011 Apr;25(8):367-70.
To develop a multiplex PCR method used to fast diagnose pathogenic fungi of fungal rhinosinusitis.
Three pairs of primers (universal fungus-specific primers, aspergillus specific primers, mucor specific primers)were used to establish multiplex PCR system. Detected six important medical fungi species strains to examine the applicability of the system and explore the optimum conditions. Multiplex PCR system was also used to identify pathogenic fungi of fungal rhinosinusitis, and used to compare the sensitivity with fungal culture.
Three pairs of primers of the multiplex PCR system had high versatility and specificity, and could amplify the corresponding DNA fragments in the appropriate conditions. Sensitivity of the reaction system was 10 microg/L. Twenty clinical specimens of fungal sinusitis were tested by fungal culture and the multiplex PCR system, 7 (35%) cases were culture positive whereas 15 (75%) cases were positive by the multiplex PCR system, the difference was statistically significant between the two methods (P < 0.05).
The multiplex PCR system was proved to be a rapid, sensitive and specific technology, it could be used to detect and identify clinical pathogenic fungi of fungal rhinosinusitis.
建立一种用于快速诊断真菌性鼻窦炎致病真菌的多重聚合酶链反应（PCR）方法。
使用三对引物（通用真菌特异性引物、曲霉特异性引物、毛霉特异性引物）建立多重PCR体系。检测六种重要的医学真菌菌株，以检验该体系的适用性并探索最佳条件。多重PCR体系还用于鉴定真菌性鼻窦炎的致病真菌，并与真菌培养法比较敏感性。
多重PCR体系的三对引物具有高度的通用性和特异性，能在合适条件下扩增出相应的DNA片段。反应体系的敏感性为10微克/升。采用真菌培养法和多重PCR体系检测20份真菌性鼻窦炎临床标本，真菌培养法阳性7例（35%），多重PCR体系阳性15例（75%），两种方法差异有统计学意义（P<0.05）。
多重PCR体系是一种快速、敏感且特异的技术，可用于检测和鉴定真菌性鼻窦炎的临床致病真菌。
