用于快速、准确鉴定真菌培养物的菌落多重串联PCR
Colony multiplex-tandem PCR for rapid, accurate identification of fungal cultures.
作者信息
Lau Anna, Sorrell Tania C, Lee OkCha, Stanley Keith, Halliday Catriona
机构信息
Centre for Infectious Diseases and Microbiology, Westmead Hospital, Westmead, New South Wales, Australia.
出版信息
J Clin Microbiol. 2008 Dec;46(12):4058-60. doi: 10.1128/JCM.01411-08. Epub 2008 Oct 22.
Abstract
We developed a multiplex tandem PCR (MT-PCR) assay for the rapid identification of 16 fungi directly from culture. MT-PCR results were concordant with phenotypic identification for all cultures studied (n = 183). The colony MT-PCR assay was rapid (<2 h), sensitive, and specific in identifying fungal pathogens directly from primary isolation plates.
摘要
我们开发了一种多重串联PCR（MT-PCR）检测方法，用于直接从培养物中快速鉴定16种真菌。MT-PCR结果与所有研究培养物（n = 183）的表型鉴定结果一致。菌落MT-PCR检测方法快速（<2小时）、灵敏且特异，可直接从初次分离平板中鉴定真菌病原体。
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