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本文引用的文献

1
Multiplex tandem PCR: a novel platform for rapid detection and identification of fungal pathogens from blood culture specimens.多重串联PCR：一种用于从血培养标本中快速检测和鉴定真菌病原体的新型平台。
J Clin Microbiol. 2008 Sep;46(9):3021-7. doi: 10.1128/JCM.00689-08. Epub 2008 Jul 16.
2
DNA microarray based on arrayed-primer extension technique for identification of pathogenic fungi responsible for invasive and superficial mycoses.基于阵列引物延伸技术的DNA微阵列用于鉴定引起侵袭性和浅表性真菌病的致病真菌。
J Clin Microbiol. 2008 Mar;46(3):909-15. doi: 10.1128/JCM.01406-07. Epub 2007 Dec 26.
3
Multiplex PCR identification of eight clinically relevant Candida species.八种临床相关念珠菌属菌种的多重聚合酶链反应鉴定
Med Mycol. 2007 Nov;45(7):619-27. doi: 10.1080/13693780701501787.
4
Rapid identification of pathogenic yeast isolates by real-time PCR and two-dimensional melting-point analysis.通过实时聚合酶链反应和二维熔点分析快速鉴定致病性酵母分离株
Eur J Clin Microbiol Infect Dis. 2007 Nov;26(11):813-8. doi: 10.1007/s10096-007-0369-2.
5
High-throughput identification of clinical pathogenic fungi by hybridization to an oligonucleotide microarray.通过与寡核苷酸微阵列杂交对临床致病真菌进行高通量鉴定。
J Clin Microbiol. 2006 Sep;44(9):3299-305. doi: 10.1128/JCM.00417-06.
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Identification of medically important yeast species by sequence analysis of the internal transcribed spacer regions.通过内部转录间隔区序列分析鉴定医学上重要的酵母菌种。
J Clin Microbiol. 2006 Mar;44(3):693-9. doi: 10.1128/JCM.44.3.693-699.2006.
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Candida bracarensis sp. nov., a novel anamorphic yeast species phenotypically similar to Candida glabrata.布拉卡假丝酵母新种，一种表型与光滑假丝酵母相似的新型无性型酵母菌种。
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9
Multiplexed tandem PCR: gene profiling from small amounts of RNA using SYBR Green detection.多重串联PCR：使用SYBR Green检测法对少量RNA进行基因分析。
Nucleic Acids Res. 2005 Nov 24;33(20):e180. doi: 10.1093/nar/gni182.
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Development of a DNA microarray for detection and identification of fungal pathogens involved in invasive mycoses.用于检测和鉴定侵袭性真菌病相关真菌病原体的DNA微阵列的研发。
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用于快速、准确鉴定真菌培养物的菌落多重串联PCR

Colony multiplex-tandem PCR for rapid, accurate identification of fungal cultures.

作者信息

Lau Anna, Sorrell Tania C, Lee OkCha, Stanley Keith, Halliday Catriona

机构信息

Centre for Infectious Diseases and Microbiology, Westmead Hospital, Westmead, New South Wales, Australia.

出版信息

J Clin Microbiol. 2008 Dec;46(12):4058-60. doi: 10.1128/JCM.01411-08. Epub 2008 Oct 22.

DOI:10.1128/JCM.01411-08
PMID:18945844
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2593288/
Abstract

We developed a multiplex tandem PCR (MT-PCR) assay for the rapid identification of 16 fungi directly from culture. MT-PCR results were concordant with phenotypic identification for all cultures studied (n = 183). The colony MT-PCR assay was rapid (<2 h), sensitive, and specific in identifying fungal pathogens directly from primary isolation plates.

摘要

我们开发了一种多重串联PCR（MT-PCR）检测方法，用于直接从培养物中快速鉴定16种真菌。MT-PCR结果与所有研究培养物（n = 183）的表型鉴定结果一致。菌落MT-PCR检测方法快速（<2小时）、灵敏且特异，可直接从初次分离平板中鉴定真菌病原体。