蛋白质修饰纳米管的可逆阳离子响应。
Reversible cation response with a protein-modified nanopipette.
出版信息
Anal Chem. 2011 Aug 15;83(16):6121-6. doi: 10.1021/ac201322v. Epub 2011 Jul 22.
Abstract
The calcium ion response of a quartz nanopipette was enhanced by immobilization of calmodulin to the nanopore surface. Binding to the analyte is rapidly reversible in neutral buffer and requires no change in media or conditions to regenerate the receptor. The signal remained reproducible over numerous measurements. The modified nanopipette was used to measure binding affinity to calcium ions, with a K(d) of 6.3 ± 0.8 × 10(-5) M. This affinity is in good agreement with reported values of the solution-state protein. The behavior of such reversible nanopore-based sensors can be used to study proteins in a confined environment and may lead to new devices for continuous monitoring.
摘要
钙调蛋白固定在纳米孔表面可以增强石英纳米管中的钙离子响应。在中性缓冲液中，与分析物的结合是快速可逆的，不需要改变介质或条件来再生受体。该信号在多次测量中仍然具有可重复性。修饰后的纳米管用于测量对钙离子的结合亲和力，其 K(d)为 6.3 ± 0.8 × 10(-5) M。该亲和力与溶液状态蛋白的报道值吻合良好。这种基于可逆纳米孔的传感器的行为可用于研究受限环境中的蛋白质，并可能为连续监测开发新设备。
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