Department of Chemical and Petroleum Engineering, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, USA.
ACS Nano. 2011 Aug 23;5(8):6736-42. doi: 10.1021/nn2023107. Epub 2011 Jul 19.
Enzyme-assisted bottom-up nanofabrication has attracted considerable attention because it provides an ideal strategy for fabricating and tailoring well-defined nanostructures with desired properties under physiological reaction conditions. Here, we report self-assembly and transformation of nanostructures controlled by enzymatic kinetics in a system consisting of horseradish peroxidase (HRP), hydrogen peroxide (H(2)O(2)), and 3,3',5,5'-tetramethylbenzidine (TMB). In this system, several TMB derivatives, produced in the presence of the enzyme HRP at different concentrations, assemble into nanoscale structures in a variety of morphologies and colors. Significantly, the assembly process is reversible, resulting in transformation of the synthesized nanostructures from one morphology into another through disassembly and reassembly processes under physiological conditions. The capability of synthesizing and controlling these "nano-transformers" through tuning enzymatic kinetics creates new opportunities for synthesis of smart materials and biomimetic nanofabrication.
酶辅助的自下而上的纳米制造引起了相当大的关注，因为它提供了一种理想的策略，可在生理反应条件下制造和调整具有所需性质的明确定义的纳米结构。在这里，我们报告了在包含辣根过氧化物酶（HRP）、过氧化氢（H(2)O(2)）和 3,3',5,5'-四甲基联苯胺（TMB）的体系中，受酶动力学控制的纳米结构的自组装和转变。在该体系中，在不同浓度的酶 HRP 的存在下生成的几种 TMB 衍生物以各种形态和颜色组装成纳米级结构。重要的是，组装过程是可逆的，通过在生理条件下的解组装和再组装过程，导致合成的纳米结构从一种形态转变为另一种形态。通过调整酶动力学来合成和控制这些“纳米变形金刚”的能力为合成智能材料和仿生纳米制造创造了新的机会。
