Winters T A, Williams M V
Department of Medical Microbiology and Immunology, Ohio State University, Columbus 43210.
J Virol Methods. 1990 Sep;29(3):233-42. doi: 10.1016/0166-0934(90)90051-g.
The bacteriophage PBS2 encoded uracil-DNA glycosylase (UNG) inhibitor was examined for its effect upon the nuclear UNG activities of KB, HeLa, and Vero cells infected with herpes simplex virus (HSV) type 1 or 2 and mock-infected cells. UNG activity from HSV-1 infected cells exhibited the greatest sensitivity to inhibition by the inhibitor, while UNG activity from cells infected with HSV-2 exhibited the greatest resistance. This differential effect was dependent upon the virus, cell line, and buffer system used in the reaction. Furthermore, the PBS2 UNG inhibitor's differential effect, provides a means of distinguishing the herpesvirus UNG activities from one another, and from the cellular UNG activity. Therefore, this method of identification should prove to be useful for the purification and characterization of the viral enzymes from infected cell nuclear extracts.
对噬菌体PBS2编码的尿嘧啶-DNA糖基化酶（UNG）抑制剂进行了检测，以研究其对感染1型或2型单纯疱疹病毒（HSV）的KB、HeLa和Vero细胞以及模拟感染细胞的细胞核UNG活性的影响。来自HSV-1感染细胞的UNG活性对该抑制剂的抑制最为敏感，而来自HSV-2感染细胞的UNG活性则表现出最大的抗性。这种差异效应取决于反应中使用的病毒、细胞系和缓冲系统。此外，PBS2 UNG抑制剂的差异效应提供了一种区分疱疹病毒UNG活性彼此之间以及与细胞UNG活性的方法。因此，这种鉴定方法对于从感染细胞核提取物中纯化和表征病毒酶应该是有用的。
