National Key Facility for Crop Gene Resources and Genetic Improvement, Ministry of Agriculture, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Haidian District, Beijing 100081, China.
J Integr Plant Biol. 2011 Sep;53(9):719-30. doi: 10.1111/j.1744-7909.2011.01067.x.
Carbon isotope discrimination (Δ(13)C) is considered a useful indicator for indirect selection of grain yield (GY) in cereals. Therefore, it is important to evaluate the genetic variation in Δ(13)C and its relationship with GY. A doubled haploid (DH) population derived from a cross of two common wheat varieties, Hanxuan 10 (H10) and Lumai 14 (L14), was phenotyped for Δ(13)C in the flag leaf, GY and yield associated traits in two trials contrasted by water availability, specifically, rain-fed and irrigated. Quantitative trait loci (QTLs) were identified by single locus and two locus QTL analyses. QTLs for Δ(13)C were located on chromosomes 1A, 2B, 3B, 5A, 7A and 7B, and QTLs for other traits on all chromosomes except 1A, 4D, 5A, 5B and 6D. The population selected for high Δ(13)C had an increased frequency of QTL for high Δ(13)C, GY and number of spikes per plant (NSP) when grown under rain-fed conditions and only for high Δ(13)C and NSP when grown under irrigated conditions, which was consistent with agronomic performance of the corresponding trait values in the high Δ(13)C progeny; that is, significantly greater than that in the low Δ(13)C. Therefore, selection for Δ(13)C was beneficial in increasing grain yield in rain-fed environments.
碳同位素分馏（Δ(13)C）被认为是间接选择谷物产量（GY）的有用指标。因此，评估 Δ(13)C 的遗传变异及其与 GY 的关系非常重要。从两个普通小麦品种韩选 10（H10）和鲁麦 14（L14）杂交衍生的双单倍体（DH）群体在两个试验中对旗叶 Δ(13)C、GY 和产量相关性状进行表型分析，这两个试验的水分可利用性不同，具体为雨养和灌溉。通过单基因座和双基因座 QTL 分析鉴定数量性状基因座（QTL）。Δ(13)C 的 QTL 位于染色体 1A、2B、3B、5A、7A 和 7B 上，其他性状的 QTL 位于除 1A、4D、5A、5B 和 6D 以外的所有染色体上。在雨养条件下，选择高 Δ(13)C 的群体具有增加高 Δ(13)C、GY 和每株穗数（NSP）的 QTL 的频率，而在灌溉条件下仅具有高 Δ(13)C 和 NSP 的 QTL，这与高 Δ(13)C 后代相应性状值的农艺表现一致；即，显著大于低 Δ(13)C。因此，在雨养环境中选择 Δ(13)C 有利于增加谷物产量。
