State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
J Integr Plant Biol. 2012 Jan;54(1):33-44. doi: 10.1111/j.1744-7909.2011.01088.x. Epub 2011 Dec 29.
In order to explore the genetics of dark-induced senescence in winter wheat (Triticum aestivum L.), a quantitative trait loci (QTL) analysis was carried out in a doubled haploid population developed from a cross between the varieties Hanxuan 10 (HX) and Lumai 14 (LM). The senescence parameters chlorophyll content (Chl a+b, Chl a, and Chl b), original fluorescence (Fo), maximum fluorescence level (Fm), maximum photochemical efficiency (Fv/Fm), and ratio of variable fluorescence to original fluorescence (Fv/Fo) were evaluated in the second leaf of whole three-leaf seedlings subjected to 7 d of darkness. A total of 43 QTLs were identified that were associated with dark-induced senescence using composite interval mapping. These QTLs were mapped to 20 loci distributed on 11 chromosomes: 1B, 1D, 2A, 2B, 3B, 3D, 5D, 6A, 6B, 7A, and 7B. The phenotypic variation explained by each QTL ranged from 7.5% to 19.4%. Eleven loci coincided with two or more of the analyzed parameters. In addition, 14 loci co-located or were linked with previously reported QTLs regulating flag leaf senescence, tolerance to high light stress, and grain protein content (Gpc), separately.
为了探索冬小麦（Triticum aestivum L.）暗诱导衰老的遗传基础，利用汉选 10（HX）和鲁麦 14（LM）杂交衍生的双单倍体群体进行了数量性状位点（QTL）分析。在三叶期幼苗的第二叶中，评估了 7 天黑暗处理后叶绿素含量（Chl a+b、Chl a 和 Chl b）、原始荧光（Fo）、最大荧光水平（Fm）、最大光化学效率（Fv/Fm）和可变荧光与原始荧光的比值（Fv/Fo）等衰老参数。利用复合区间作图法共鉴定到 43 个与暗诱导衰老相关的 QTL。这些 QTL 被定位到 20 个位于 11 条染色体上的位点：1B、1D、2A、2B、3B、3D、5D、6A、6B、7A 和 7B。每个 QTL 解释的表型变异幅度为 7.5%至 19.4%。11 个位点与两个或更多分析参数重合。此外，14 个位点与先前报道的调控旗叶衰老、高光胁迫耐受性和籽粒蛋白质含量（Gpc）的 QTL 共定位或连锁。
