Laboratory of Protozoology, Institute of Evolution & Marine Biodiversity, Ocean University of China, Qingdao 266003, China.
J Eukaryot Microbiol. 2011 Sep-Oct;58(5):437-45. doi: 10.1111/j.1550-7408.2011.00567.x. Epub 2011 Jul 18.
The morphology, infraciliature, and molecular phylogeny of Pseudodiophrys nigricans n. g., n. sp., and Paradiophrys zhangi n. sp., isolated from a sandy beach near Qingdao, China, were investigated. Pseudodiophrys is characterized by the Diophrys-like ciliature pattern, but having only a single, reduced undulating membrane. Pseudodiophrys nigricans, the type species by monotypy, is described from live and silver-impregnated specimens. Paradiophrys zhangi is similar to the type species Paradiophrys irmgard but can be recognized by its border body and the number (7 vs. 8-10) and arrangement (in rows vs. sparsely distributed) of the frontoventral cirri. Small subunit (SSU) rRNA gene sequence data support the validity of both species. Phylogenetic analyses based on the SSU rRNA gene sequence data currently available for uronychiids and other related taxa indicate that P. nigricans is most closely related to Diophrys scutum and Diophrys apoligothrix, while P. zhangi clusters most closely with Apodiophrys ovalis within a clade that also includes two other Paradiophrys species.
本研究调查了采自中国青岛附近沙滩的 Pseudodiophrys nigricans n. g., n. sp. 和 Paradiophrys zhangi n. sp. 的形态、纤毛结构和分子系统发育。Pseudodiophrys 的特征为具 Diophrys 样纤毛结构，但仅有单一的、退化的波动膜。单型种 Pseudodiophrys nigricans 基于活体和银染标本进行了描述。Paradiophrys zhangi 与模式种 Paradiophrys irmgard 相似，但可通过额前纤毛的体环和数量（7 对 8-10）及排列（成行 vs. 稀疏分布）来区分。小亚基（SSU）rRNA 基因序列数据支持这两个物种的有效性。基于目前可获得的关于游仆虫目和其他相关类群的 SSU rRNA 基因序列数据的系统发育分析表明，P. nigricans 与 Diophrys scutum 和 Diophrys apoligothrix 的亲缘关系最密切，而 P. zhangi 与 Apodiophrys ovalis 聚类最密切，该分支还包括另外两个 Paradiophrys 物种。
