Department of Botany, Oklahoma State University, Stillwater, OK 74078, USA.
Virus Res. 2013 Sep;176(1-2):161-8. doi: 10.1016/j.virusres.2013.06.001. Epub 2013 Jun 14.
A new panicovirus was identified from a metagenomic survey of plant viruses in the Tallgrass Prairie Preserve (TPP), Osage County, Oklahoma, USA. Thin paspalum asymptomatic virus (TPAV) was isolated from Paspalum setaceum var. muehlenbergii. The complete 4195 nucleotide sequence encodes six proteins of 44, 109, 8, 6.8, 26, and 15kDa, whose genes are similar in size and location to those of panicum mosaic virus (PMV) and cocksfoot mild mosaic virus (CMMV). Infectious transcripts made from cDNAs of the 2005 isolate were used for initial host range analyses. Of the 11 tested grasses, only Paspalum setaceum (var. stramineum), Setaria italica, and Setaria viridis were hosts, producing asymptomatic infections, and Nicotiana benthamiana was not infected. Protein and RNA alignments indicate that TPAV is more closely related to PMV than CMMV. RNA alignments and structure predictions indicate that TPAV and CMMV have conserved eight structural elements previously predicted or analyzed for PMV. The initial TPP specimen also contained RNAs related to alphacryptoviruses. Partial sequences of RNA1 (∼35%) and RNA2 (∼40%) of putative thin paspalum cryptic virus were obtained. These represent the first sequences for a putative grass-infecting alphacryptovirus.
一种新的弹状病毒是从美国俄克拉荷马州奥塞奇县塔尔斯草甸保护区（TPP）的植物病毒宏基因组调查中鉴定出来的。薄叶雀稗无症状病毒（TPAV）是从雀稗的一个变种中分离出来的。完整的 4195 个核苷酸序列编码六个蛋白质，分子量分别为 44、109、8、6.8、26 和 15kDa，其基因的大小和位置与雀稗花叶病毒（PMV）和鸡眼草中度花叶病毒（CMMV）相似。来自 2005 年分离株 cDNA 的传染性转录本用于初步的宿主范围分析。在测试的 11 种禾本科植物中，只有雀稗（var. stramineum）、狗尾草和野燕麦是宿主，产生无症状感染，而不感染烟草。蛋白质和 RNA 比对表明，TPAV 与 PMV 的亲缘关系比 CMMV 更密切。RNA 比对和结构预测表明，TPAV 和 CMMV 具有保守的 8 个结构元件，这些元件以前被预测或分析过 PMV。最初的 TPP 标本还含有与α- cryptoviruses 相关的 RNA。获得了推定的薄叶雀稗隐性病毒 RNA1（约 35%）和 RNA2（约 40%）的部分序列。这代表了第一个推定的感染草的α- cryptovirus 的序列。
