Medical Research Center, Taizhou Hospital of Zhejiang Province, Wenzhou Medical College, Linhai, Zhejiang, China.
Hum Immunol. 2011 Nov;72(11):1068-73. doi: 10.1016/j.humimm.2011.06.012. Epub 2011 Jul 1.
The significance of upregulated soluble human leukocyte antigen-G (sHLA-G) expression under various pathologic conditions has been discussed. In this study, we evaluated the potential significance of plasma sHLA-G expression in patients with hepatitis B virus (HBV) infection. The study included 90 acute hepatitis B patients (AHB), 131 chronic hepatitis B patients (CHB), 152 resolved hepatitis B individuals (RHB), and 129 normal controls. sHLA-G were determined using enzyme-linked immunosorbent assay. A receiver operating characteristic (ROC) curve was used to evaluate the feasibility of plasma sHLA-G as a biomarker for distinguishing patients with HBV infection. sHLA-G levels in AHB (median, 193.1 U/mL; p < 0.001), CHB (median, 324.6 U/mL; p < 0.001), and RHB (median, 14.8 U/mL; p = 0.006) patients was much higher than that in normal controls (median, 9.0 U/mL). A significant difference for sHLA-G levels was also observed between patients with HBV infection (AHB vs CHB, AHB vs RHB, and CHB vs RHB; all p < 0.001). The area under the ROC curve for sHLA-G levels was 1.000 (p < 0.001) for AHB, 0.993 (p < 0.001) for CHB, and 0.604 (p = 0.003) for RHB patients versus normal controls, respectively. Data also indicated that the percentage of CD4(+)CD25(+)FoxP3(+) T regulatory cells and HLA-G(+)CD14(+) monocytes was significantly increased in AHB and CHB patients compared with normal controls (all p < 0.001). Our findings indicated that induction of HLA-G expression may play a role in HBV immune evasion and sHLA-G levels could be a useful biomarker in HBV infection.
在各种病理条件下，上调可溶性人类白细胞抗原-G（sHLA-G）表达的意义已经被讨论过了。在这项研究中，我们评估了血浆 sHLA-G 表达在乙型肝炎病毒（HBV）感染患者中的潜在意义。研究包括 90 例急性乙型肝炎患者（AHB）、131 例慢性乙型肝炎患者（CHB）、152 例乙型肝炎已恢复患者（RHB）和 129 例正常对照者。使用酶联免疫吸附试验（ELISA）测定 sHLA-G。使用受试者工作特征（ROC）曲线评估血浆 sHLA-G 作为区分 HBV 感染患者的生物标志物的可行性。AHB（中位数，193.1 U/mL；p < 0.001）、CHB（中位数，324.6 U/mL；p < 0.001）和 RHB（中位数，14.8 U/mL；p = 0.006）患者的 sHLA-G 水平明显高于正常对照组（中位数，9.0 U/mL）。HBV 感染患者（AHB 与 CHB、AHB 与 RHB 和 CHB 与 RHB）之间的 sHLA-G 水平也存在显著差异（均 p < 0.001）。ROC 曲线下面积（AUC）sHLA-G 水平在 AHB 患者中为 1.000（p < 0.001），在 CHB 患者中为 0.993（p < 0.001），在 RHB 患者中为 0.604（p = 0.003），与正常对照组相比。数据还表明，与正常对照组相比，AHB 和 CHB 患者的 CD4(+)CD25(+)FoxP3(+)T 调节细胞和 HLA-G(+)CD14(+)单核细胞的百分比显著增加（均 p < 0.001）。我们的研究结果表明，HLA-G 表达的诱导可能在乙型肝炎病毒免疫逃逸中发挥作用，sHLA-G 水平可能是乙型肝炎病毒感染的有用生物标志物。
