嗜麦芽寡养单胞菌在囊性纤维化中的检测：选择性琼脂的应用提高了检测率，并评估了其抗菌药物耐药性。
Stenotrophomonas maltophilia in cystic fibrosis: improved detection by the use of selective agar and evaluation of antimicrobial resistance.
机构信息
Institute of Medical Microbiology, University Hospital Essen, University of Duisburg-Essen, Essen, Germany.
出版信息
J Cyst Fibros. 2011 Dec;10(6):422-7. doi: 10.1016/j.jcf.2011.06.010. Epub 2011 Jul 16.
BACKGROUND
We implemented a selective medium for improved detection of Stenotrophomonas maltophilia in sputum samples from CF patients. We also performed antimicrobial susceptibility testing with eight antibiotics.
METHODS
A total of 623 consecutive sputum samples from 165 CF patients in a German CF center were cultured onto conventional media and onto Steno medium agar (SMA). All isolates confirmed as S. maltophilia by biochemical and molecular methods were subjected to antimicrobial susceptibility testing. The following agents were tested by Etest: ceftazidime, levofloxacin, moxifloxacin, tigecycline, trimethoprim-sulfamethoxazole, fosfomycin, colistin, and ticarcillin-clavulanate acid.
RESULTS
Conventional media supported the growth of S. maltophilia in 7.1% of samples, whereas SMA supported its growth in 11.6%, increasing the detection rate to 64%. Trimethoprim-sulfamethoxazole and tigecycline exhibited the highest in vitro activity, whereas ceftazidime, colistin, and ticarcillin-clavulanate acid exhibited higher resistance rates.
CONCLUSIONS
SMA is a promising medium allowing improved isolation of S. maltophilia from sputum samples from CF patients. Trimethoprim-sulfamethoxazole and tigecycline demonstrated excellent inhibitory effects against S. maltophilia, which may suggest a potential clinical effect.
背景
我们开发了一种选择性培养基，以提高 CF 患者痰液样本中嗜麦芽寡养单胞菌的检出率。我们还对 8 种抗生素进行了药敏试验。
方法
对德国 CF 中心的 165 例 CF 患者的 623 份连续痰液样本进行培养，分别接种于常规培养基和 Steno 培养基琼脂（SMA）。采用生化和分子方法确认的所有嗜麦芽寡养单胞菌分离株均进行药敏试验。采用 Etest 法检测以下药物：头孢他啶、左氧氟沙星、莫西沙星、替加环素、复方磺胺甲噁唑、磷霉素、多粘菌素和替卡西林克拉维酸。
结果
常规培养基支持嗜麦芽寡养单胞菌生长的比例为 7.1%，而 SMA 支持其生长的比例为 11.6%，从而将检出率提高至 64%。复方磺胺甲噁唑和替加环素具有最高的体外活性，而头孢他啶、多粘菌素和替卡西林克拉维酸的耐药率较高。
结论
SMA 是一种有前途的培养基，可提高 CF 患者痰液样本中嗜麦芽寡养单胞菌的分离率。复方磺胺甲噁唑和替加环素对嗜麦芽寡养单胞菌具有良好的抑制作用，这可能提示具有潜在的临床效果。
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