嗜麦芽窄食单胞菌的基因操作
Genetic Manipulation of Stenotrophomonas maltophilia.
作者信息
Welker Elliott, Domfeh Yayra, Tyagi Deepti, Sinha Sanjivni, Fisher Nathan
机构信息
North Dakota State University, Department of Veterinary and Microbiological Sciences, Fargo, North Dakota.
出版信息
Curr Protoc Microbiol. 2015 May 1;37:6F.2.1-14. doi: 10.1002/9780471729259.mc06f02s37.
Abstract
Stenotrophomonas maltophilia is a Gram-negative, aerobic, motile, environmental bacterium that is emerging as an important nosocomial pathogen with high rates of attributable mortality in severely ill patients. S. maltophilia is of particular concern to patients suffering from cystic fibrosis (CF) as it has been shown to colonize airway epithelial and establish a chronic infection. Here we describe several molecular techniques for the genetic manipulation of this bacterium, including DNA extraction, RNA extraction, conjugation of plasmids from Escherichia coli and allelic exchange.
摘要
嗜麦芽窄食单胞菌是一种革兰氏阴性、需氧、可运动的环境细菌，正逐渐成为一种重要的医院病原体，在重症患者中导致较高的可归因死亡率。嗜麦芽窄食单胞菌尤其引起囊性纤维化（CF）患者的关注，因为已证明它可定殖于气道上皮并建立慢性感染。在此，我们描述了几种用于该细菌基因操作的分子技术，包括DNA提取、RNA提取、从大肠杆菌进行质粒接合以及等位基因交换。
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