Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, CEP 31270-901, Belo Horizonte-MG, Brazil.
Peptides. 2011 Aug;32(8):1640-6. doi: 10.1016/j.peptides.2011.06.028. Epub 2011 Jul 6.
Mutalysin-II (mut-II) from Lachesis muta snake venom is an endopeptidase with hemorrhagic activity. A mAb against mutalysin-II that neutralized the hemorrhagic effect was produced previously. To identify the mAb epitopes, sets of 15-mer overlapping peptides covering the mut-II amino acid sequence were synthesized using the SPOT method and tested but failed to react with the mAb. Using a phage-display approach seventeen clones reactive with mAb were identified. Additional immunoassays with the peptides and mAb identified the QCTMDQGRLRCR, TCATDQGRLRCT, HCFHDQGRVRCA, HCTMDQGRLRCR and SCMLDQGRSRCR sequences as possible epitopes. Immunization of rabbits with these peptides induced antibodies that recognize mut-II and protected against the hemorrhagic effects of Lachesis venom.
来自美拉蛇毒的Mutalysin-II（mut-II）是一种具有出血活性的内肽酶。之前已经产生了一种针对 mutalysin-II 的中和出血作用的 mAb。为了鉴定 mAb 表位，使用 SPOT 方法合成了覆盖 mut-II 氨基酸序列的 15 个重叠肽的集合，并进行了测试，但未能与 mAb 反应。使用噬菌体展示方法，鉴定出 17 个与 mAb 反应的克隆。使用肽和 mAb 的额外免疫测定鉴定出 QCTMDQGRLRCR、TCATDQGRLRCT、HCFHDQGRVRCA、HCTMDQGRLRCR 和 SCMLDQGRSRCR 序列作为可能的表位。用这些肽免疫兔子诱导了识别 mut-II 并防止美拉蛇毒液出血作用的抗体。
