Southeast Asian Ministers of Education Organization (SEAMEO), Regional Center For Food and Nutrition (RECFON), University of Indonesia, 6 Salemba Raya, Jakarta 10430, Indonesia.
Anaerobe. 2011 Dec;17(6):496-500. doi: 10.1016/j.anaerobe.2011.06.003. Epub 2011 Jul 5.
Enterococcus faecium IS-27526 is a novel probiotic isolated from dadih, an Indonesian traditional fermented buffalo milk. A 90 days randomized double-blind placebo-controlled study of pre-post trial was conducted in pre-school children with two groups, placebo and probiotic group. Ultra High Temperature low fat milk was used as a carrier in each group. The aims of this study were to investigate the effect of E. faecium IS-27526 in milk on humoral immune response and on bodyweight of pre-school children. Total serum IgA and total salivary sIgA were measured by sandwich ELISA. The bodyweight of young children was measured. The results showed that total serum IgA did not significantly increase in the probiotic group compared with the placebo group. Total salivary sIgA level and the bodyweight significantly increased (p < 0.05) in probiotic groups compared to the placebo. Changes of total salivary sIgA level were significantly higher in underweight children supplemented with probiotic. Weight gain was observed significantly in children with normal bodyweight supplemented with probiotic. Neither mortality nor weight loss was recorded throughout the study. Taken together, novel probiotic E. faecium IS-27526 has significant positive effects on humoral immune response, salivary sIgA, in underweight pre-school children, and on weight gain of pre-school children.
屎肠球菌 IS-27526 是从印度尼西亚传统发酵水牛乳凝乳中分离出来的一种新型益生菌。一项为期 90 天的随机双盲安慰剂对照研究在学龄前儿童中进行，分为安慰剂组和益生菌组。每组均使用超高温低脂牛奶作为载体。本研究旨在研究 E. faecium IS-27526 对学龄前儿童体液免疫反应和体重的影响。采用夹心 ELISA 法检测总血清 IgA 和总唾液 sIgA。测量幼儿的体重。结果表明，与安慰剂组相比，益生菌组总血清 IgA 无显著增加。与安慰剂组相比，益生菌组的总唾液 sIgA 水平和体重显著增加（p <0.05）。补充益生菌的体重不足儿童的总唾液 sIgA 水平变化显著升高。补充益生菌的正常体重儿童体重增加明显。整个研究过程中未记录到死亡率或体重减轻。综上所述，新型益生菌屎肠球菌 IS-27526 对体液免疫反应、唾液 sIgA、体重不足的学龄前儿童具有显著的积极影响，并能增加学龄前儿童的体重。
