Department of Food and Agricultural Product Technology, Faculty of Agricultural Technology, Universitas Gadjah Mada, Jl. Flora No 1 Bulaksumur, Yogyakarta 55281, Indonesia.
Centre for Food and Nutrition Studies, Universitas Gadjah Mada, Jl. Teknika Utara Barek, Yogyakarta 55281, Indonesia.
Nutrients. 2022 Mar 1;14(5):1049. doi: 10.3390/nu14051049.
Undernutrition is associated with gut microbiota unbalance, and probiotics are believed to restore it and improve gut integrity. A randomized double-blind controlled trial was conducted to evaluate the efficacy of gummy Dad-13 (10 CFU/3 g) to prevent the progression of severe undernutrition. Two groups of moderate undernutrition infants were involved in this study, namely the placebo ( = 15) and probiotics ( = 15) groups, and were required to consume the product for 50 days. 16S rRNA sequencing and qPCR were used for gut microbiota analysis, and gas chromatography was used to analyze Short-Chain Fatty Acid (SCFA). The daily food intake of both groups was recorded using food records. Our results revealed that the probiotic group had better improvements regarding the anthropometry and nutritional status. In addition, Dad-13 modulated the butyric acid-producing bacteria to increase and inhibit the growth of Enterobacteriaceae. This gut modulation was associated with the increment in SCFA, especially total SCFA, propionic, and butyric acid. The number of was increased after the probiotic intervention. However, Dad-13 was not able to change the alpha and beta diversity. Therefore, Dad-13 has been proven to promote the growth of beneficial bacteria.
营养不良与肠道微生物失衡有关，益生菌被认为可以恢复肠道微生物平衡并改善肠道完整性。本研究采用随机、双盲、对照试验，旨在评估 gummy Dad-13（10 CFU/3 g）预防严重营养不良进展的疗效。本研究纳入两组中重度营养不良婴儿，即安慰剂组（n=15）和益生菌组（n=15），要求两组婴儿均连续服用该产品 50 天。采用 16S rRNA 测序和 qPCR 进行肠道微生物分析，采用气相色谱法分析短链脂肪酸（SCFA）。使用食物记录记录两组的每日食物摄入量。研究结果表明，益生菌组在人体测量和营养状况方面的改善情况更好。此外， Dad-13 调节丁酸产生菌的生长，增加丁酸产生菌的数量，抑制肠杆菌科的生长。这种肠道调节与 SCFA 的增加有关，尤其是总 SCFA、丙酸和丁酸。益生菌干预后 的数量增加。然而， Dad-13 并不能改变 alpha 和 beta 多样性。因此，Dad-13 已被证明可以促进有益菌的生长。
