Bakker-Zierikzee A M, Tol E A F, Kroes H, Alles M S, Kok F J, Bindels J G
Division of Human Nutrition, Wageningen University, Wageningen, The Netherlands.
Pediatr Allergy Immunol. 2006 Mar;17(2):134-40. doi: 10.1111/j.1399-3038.2005.00370.x.
Secretory immunoglobulin A (SIgA) plays an important role in the defence of the gastrointestinal tract. The level of faecal SIgA antibody is associated with increased neutralization and clearance of viruses. Formula-fed infants who lack the transfer of protective maternal SIgA from breast milk may benefit from strategies to support maturation of humoral immunity and endogenous production of SIgA. We aimed at studying the effects of standard, prebiotic and probiotic infant formulas on the faecal SIgA levels. At birth, infants of whom the mother had decided not to breastfeed were allocated to one of three formula groups in a randomized, double-blind fashion. Nineteen infants received standard infant formula; 19 received prebiotic formula containing a specific mixture of 0.6 g galacto-oligosaccharides (GOS)/fructo-oligosaccharides (FOS)/100 ml formula and 19 received probiotic formula containing 6.0 x 10(9) cfu Bifidobacterium animalis/100 ml formula. Faecal samples were taken on postnatal day 5, day 10, wk 4 and every 4 wk thereafter until wk 32. SIgA in faeces was determined by an enzyme-linked immunosorbent assay. During the intervention, infants fed on prebiotic formula showed a trend towards higher faecal SIgA levels compared with the standard formula-fed infants reaching statistical significance at the age of 16 wk. In contrast, infants fed on the probiotic formula showed a highly variable faecal SIgA concentration with no statistically significant differences compared with the standard formula group. Formula-fed infants may benefit from infant formulas containing a prebiotic mixture of GOS and FOS because of the observed clear tendency to increase faecal SIgA secretion. Adding viable B. animalis strain Bb-12 to infant formula did not reveal any sign for such a trend.
分泌型免疫球蛋白A（SIgA）在胃肠道防御中发挥着重要作用。粪便SIgA抗体水平与病毒中和及清除能力增强相关。缺乏母乳中保护性母体SIgA传递的配方奶喂养婴儿可能受益于支持体液免疫成熟和内源性SIgA产生的策略。我们旨在研究标准、益生元和益生菌婴儿配方奶粉对粪便SIgA水平的影响。出生时，母亲决定不进行母乳喂养的婴儿以随机、双盲方式被分配到三个配方奶组之一。19名婴儿接受标准婴儿配方奶粉；19名婴儿接受含有0.6克低聚半乳糖（GOS）/低聚果糖（FOS）/100毫升配方奶特定混合物的益生元配方奶粉，19名婴儿接受含有6.0×10⁹ 菌落形成单位（cfu）动物双歧杆菌/100毫升配方奶的益生菌配方奶粉。在出生后第5天、第10天、第4周以及此后每4周直至第32周采集粪便样本。通过酶联免疫吸附测定法测定粪便中的SIgA。在干预期间，与标准配方奶喂养的婴儿相比，食用益生元配方奶粉的婴儿粪便SIgA水平有升高趋势，在16周龄时达到统计学显著性。相比之下，食用益生菌配方奶粉的婴儿粪便SIgA浓度变化很大，与标准配方奶组相比无统计学显著差异。由于观察到粪便SIgA分泌明显有增加的趋势，配方奶喂养的婴儿可能从含有GOS和FOS益生元混合物的婴儿配方奶粉中受益。向婴儿配方奶粉中添加活的动物双歧杆菌Bb - 12菌株未显示出这种趋势的任何迹象。
