National Academy of Agricultural Science, Rural Development Administration, Suwon 440-707, Republic of Korea.
Plant Sci. 2011 Sep;181(3):316-23. doi: 10.1016/j.plantsci.2011.06.007. Epub 2011 Jun 14.
The WRKY proteins are a major family of plant transcription factors implicated in the regulation of plant defense mechanisms against pathogens. OsWRKY6 was isolated based on expression profiling data carried out with samples infected by Xanthomonas oryzae pv. oryzae (Xoo). OsWRKY6 encodes a DNA binding protein that contains one WRKY domain, a nuclear localization signal and C(2)H(2)-type zinc finger motif. OsWRKY6 is a member of the group II family of WRKY proteins. Based on the result of yeast one hybrid assay this OsWRKY6 protein binds to the typical W box ((T)TGACC/T). OsWRKY6 functions as a transcriptional activator in yeast. OsWRKY6 enhanced the expression of the reporter gene downstream of OsPR1 promoter, indicating that OsWRKY6 is a transcriptional activator in rice as well. Heterologous expression of OsWRKY6 enhanced disease resistance to pathogen. Defense-related genes were constitutively expressed in Arabidopsis transgenic lines overexpressing OsWRKY6. All together, OsWRKY6 functions as a positive transcriptional regulator of the plant defense response.
WRKY 蛋白是一类重要的植物转录因子，参与植物防御机制对病原体的调控。根据感染稻黄单胞菌（Xanthomonas oryzae pv. oryzae，Xoo）的样品进行的表达谱数据分析，分离出 OsWRKY6。OsWRKY6 编码一个 DNA 结合蛋白，含有一个 WRKY 结构域、一个核定位信号和 C(2)H(2)-型锌指模体。OsWRKY6 是 WRKY 蛋白家族 II 组的成员。基于酵母单杂交分析的结果，该 OsWRKY6 蛋白与典型的 W 框（(T)TGACC/T）结合。OsWRKY6 在酵母中作为转录激活因子发挥作用。OsWRKY6 增强了 OsPR1 启动子下游报告基因的表达，表明 OsWRKY6 在水稻中也是转录激活因子。OsWRKY6 的异源表达增强了对病原体的抗病性。在过表达 OsWRKY6 的拟南芥转基因系中，防御相关基因持续表达。总的来说，OsWRKY6 作为植物防御反应的正转录调控因子发挥作用。
