Australian Centre for Research on Separation Science, School of Chemistry, University of Tasmania, Hobart, Tasmania 7001, Australia.
J Chromatogr A. 2011 Aug 19;1218(33):5718-24. doi: 10.1016/j.chroma.2011.06.071. Epub 2011 Jun 28.
The performance of micelle to solvent stacking (MSS) in capillary zone electrophoresis (CZE) was improved for anionic analytes using the long chain ionic liquid type cationic surfactant 1-dodecyl-3-methylimidazolium tetrafluoroborate (C₁₂-MIM-BF₄). The peak heights and corrected peak areas of the test profens and herbicides were enhanced up to 59 and 110-fold, respectively when compared to typical injection. These were up to 10 times better compared to the surfactant cetyltrimethyl ammonium bromide as MSS carrier. This performance was attributed to the properties of C₁₂-MIM-BF₄. MSS requires micelles in the sample for transport of bound analytes to a stacking boundary that contains an organic solvent for effective electrophoretic mobility reversal. The ionic liquid micelles provided better analyte transport properties that resulted from its hydrophobic and pi-pi interaction capabilities. The good solubility of the ionic liquid in high percentages of organic solvent also facilitated a more effective reversal of mobility. The LODs obtained for the test analytes were from 0.06 to 0.12 μg/mL. The linearity R² values in terms of peak height and corrected area were ≥ 0.99. The interday repeatabilities (%RSD, n=10,) were 0.5-2.2% for retention time, 1.9-4.7% for corrected areas and 4.1-6.4% for peak heights.
使用长链离子液体型阳离子表面活性剂 1-十二烷基-3-甲基咪唑四氟硼酸盐（C12-MIM-BF4），提高了胶束电动色谱（CZE）中胶束对溶剂堆积（MSS）对阴离子分析物的性能。与典型的进样相比，测试的前体和除草剂的峰高和校正峰面积分别提高了 59 倍和 110 倍。与表面活性剂十六烷基三甲基溴化铵作为 MSS 载体相比，这要好 10 倍。这种性能归因于 C12-MIM-BF4 的性质。MSS 需要样品中的胶束来运输与结合分析物一起到包含有机溶剂的堆积边界，以实现有效的电泳迁移率反转。离子液体胶束提供了更好的分析物传输性能，这是由于其疏水性和π-π相互作用能力。离子液体在高比例有机溶剂中的良好溶解性也促进了更有效的迁移率反转。测试分析物的检出限为 0.06-0.12μg/mL。以峰高和校正面积表示的线性度 R²值均≥0.99。日内重复性（%RSD，n=10），保留时间为 0.5-2.2%，校正面积为 1.9-4.7%，峰高为 4.1-6.4%。
