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采用场放大样品进样、扫集和胶束塌陷的三步堆积毛细管电泳法测定人血浆中的达比加群及其活性代谢物。

A three-step stacking capillary electrophoresis of field-amplified sample injection, sweeping, and micellar collapse for determination of dabigatran and its active metabolite in human plasma.

机构信息

School of Pharmacy, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan, ROC.

Department of Internal Medicine, Kaohsiung Municipal Ta-Tung Hospital, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung, Taiwan, ROC.

出版信息

J Food Drug Anal. 2022 Mar 15;30(1):88-103. doi: 10.38212/2224-6614.3391.

DOI:10.38212/2224-6614.3391
PMID:35647720
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9930998/
Abstract

A three-step stacking capillary electrophoresis (CE) composed of field-amplified sample injection, sweeping, and analyte focusing by micellar collapse (FASI-sweeping-AFMC) was developed to determine dabigatran (D) and its major active metabolite, dabigatran acyl-beta-d-glucuronide (DAG), in human plasma. After optimization and validation, this novel approach was further applied to monitor 5 real samples, and the 25.2-186.8 ng mL D could be observed among those. Based on these results, the novel CE stacking strategy was successfully applied for the analysis of D and DAG in human plasma and could be served as a tool for clinical assays.

摘要

建立了一种三步堆积毛细管电泳（CE）方法，包括场放大样品进样、扫集和胶束塌陷的分析物聚焦（FASI-扫集-AFMC），用于测定人血浆中的达比加群（D）及其主要活性代谢物达比加群酰基-β-D-葡萄糖醛酸（DAG）。经过优化和验证后，该新方法进一步应用于监测 5 个真实样本，其中可观察到 25.2-186.8ngmL 的 D。基于这些结果，该新的 CE 堆积策略成功地应用于人血浆中 D 和 DAG 的分析，可以作为临床检测的工具。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8af4/9930998/b00e403a4ae2/jfda-30-88f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8af4/9930998/900ebe832182/jfda-30-88f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8af4/9930998/757f50c43df3/jfda-30-88f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8af4/9930998/f15c5869d67a/jfda-30-88f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8af4/9930998/df1b275b16e9/jfda-30-88f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8af4/9930998/b00e403a4ae2/jfda-30-88f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8af4/9930998/900ebe832182/jfda-30-88f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8af4/9930998/757f50c43df3/jfda-30-88f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8af4/9930998/f15c5869d67a/jfda-30-88f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8af4/9930998/df1b275b16e9/jfda-30-88f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8af4/9930998/b00e403a4ae2/jfda-30-88f5.jpg

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