Key Laboratory of Bioinorganic Chemistry, College of Science, Agricultural University of Hebei, Baoding, Hebei, China.
J Chromatogr B Analyt Technol Biomed Life Sci. 2012 Oct 1;906:41-7. doi: 10.1016/j.jchromb.2012.08.017. Epub 2012 Aug 21.
A sensitive method for the determination of three cationic alkaloids (berberine, palmatine and jatrorrhizine) from human plasma samples was developed by micelle to solvent stacking (MSS) in capillary zone electrophoresis (CZE). In MSS, the sample preconcentration mainly relies on the reversal in the effective electrophoretic mobility of the analytes at the boundary zone between the sample and CZE background solution (BGS). Under the optimized conditions, the sensitivity enhancement factors achieved in terms of corrected peak area were in the range from 47 to 53 for the alkaloids. The limits of detection (LODs) (S/N=3) for berberine, palmatine and jatrorrhizine were 0.01, 0.01 and 0.02μg/mL, respectively. The intraday (n=6) and interday repeatabilities (n=12) expressed as the relative standard deviations (RSDs) were less than 6.9% in terms of peak height and less than 7.3% in terms of corrected peak area, respectively. The recoveries of the method for the three alkaloids were in the range of 95.9-101.5% with peak height as the quantitative signal, and 92.6-103.6% with corrected peak area as the quantitative signal, respectively. The MSS-CZE method proved to be suitable for the analysis of the alkaloids in human plasma samples.
建立了胶束电动毛细管电泳（MEKC）中胶束增敏溶剂堆积（MSS）用于人血浆中三种阳离子型生物碱（小檗碱、巴马汀和黄连碱）同时测定的方法。在 MSS 中，样品的预浓缩主要依赖于分析物在样品与 MEKC 背景溶液（BGS）之间的边界区域内有效电泳淌度的反转。在优化条件下，生物碱的校正峰面积的灵敏度增强因子在 47 到 53 之间。小檗碱、巴马汀和黄连碱的检测限（LOD）（S/N=3）分别为 0.01、0.01 和 0.02μg/mL。日内（n=6）和日间（n=12）重复性以峰高计的相对标准偏差（RSD）小于 6.9%，以校正峰面积计的 RSD 小于 7.3%。该方法用于三种生物碱的回收率以峰高为定量信号时在 95.9-101.5%范围内，以校正峰面积为定量信号时在 92.6-103.6%范围内。MSS-CZE 法可用于人血浆中生物碱的分析。
