Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China.
Bioresour Technol. 2011 Oct;102(19):9155-61. doi: 10.1016/j.biortech.2011.06.074. Epub 2011 Jun 29.
Geobacillus pallidus XS2 and XS3 were isolated from oil contaminated soil samples in Yumen oilfield, China, and were able to produce bioemulsifiers on different hydrocarbons. Biodegradation assays exhibited that approximately 70% of PAH (250 mg/L) or 85% of crude oil (500 mg/L) was removed by the thermophilic bacteria after 20 days. The bioemulsifiers of the two strains were isolated and obtained a productive yield of 4.24±0.08 and 3.82±0.11g/L, respectively. GPC analysis revealed that the number-average molecular weights (M(n)) of the two bioemulsifiers were 271,785 Da and 526,369 Da, with PDI values of 1.104 and 1.027, respectively. Chemical composition studies exhibited that the bioemulsifier XS2 consisted of carbohydrates (68.6%), lipids (22.7%) and proteins (8.7%) while the bioemulsifier XS3 was composed by carbohydrates (41.1%), lipids (47.6%) and proteins (11.3%). Emulsification assays approved the effectiveness of bioemulsifiers over a wide range of temperature, pH and salinity.
从中国玉门油田污染土壤样本中分离得到嗜热菌 Geobacillus pallidus XS2 和 XS3，它们能够在不同的碳氢化合物上产生生物乳化剂。生物降解试验表明，经过 20 天，嗜热菌可去除约 70%的多环芳烃（250mg/L）或 85%的原油（500mg/L）。从这两株菌中分离出生物乳化剂，产量分别为 4.24±0.08 和 3.82±0.11g/L。GPC 分析表明，两种生物乳化剂的数均分子量（M(n)）分别为 271,785Da 和 526,369Da，PDI 值分别为 1.104 和 1.027。化学成分研究表明，生物乳化剂 XS2 由碳水化合物（68.6%）、脂质（22.7%）和蛋白质（8.7%）组成，而生物乳化剂 XS3 由碳水化合物（41.1%）、脂质（47.6%）和蛋白质（11.3%）组成。乳化试验证明了生物乳化剂在较宽的温度、pH 和盐度范围内的有效性。
