Biomedical and X-Ray Physics, Department of Applied Physics, KTH Royal Institute of Technology/AlbaNova, 10691 Stockholm, Sweden.
Opt Lett. 2011 Jul 15;36(14):2728-30. doi: 10.1364/OL.36.002728.
Soft-x-ray cryotomography allows quantitative and high-resolution three-dimensional imaging of intact unstained cells. To date, the method relies on synchrotron-radiation sources, which limits accessibility for researchers. Here we present a laboratory water-window microscope for cryotomography. It is based on a λ=2.48 nm liquid-jet laser-plasma source, a normal-incidence multilayer condenser, a 30 nm zone-plate objective, and a cryotilt sample holder. We demonstrate high-resolution imaging, as well as quantitative tomographic imaging, of frozen intact cells. The reconstructed tomogram of the intracellular local absorption coefficient shows details down to ∼100 nm.
软 X 射线断层摄影术允许对未经染色的完整细胞进行定量和高分辨率的三维成像。迄今为止，该方法依赖于同步辐射源，这限制了研究人员的可及性。在这里，我们展示了一种用于断层摄影术的实验室水窗显微镜。它基于 λ=2.48nm 液体射流激光等离子体源、正入射多层聚光镜、30nm 波带片物镜和冷冻倾斜样品架。我们证明了冷冻完整细胞的高分辨率成像和定量断层成像。重建的细胞内局部吸收系数断层图像显示了低至约 100nm 的细节。
