Yoshida Toru, Arii Yasuhiro, Hino Katsuhiko, Sawatani Ikuo, Tanaka Midori, Takahashi Rei, Bando Toru, Mukai Kazuhisa, Fukuo Keisuke
Department of Food Science and Nutrition, School of Human Environmental Sciences, Mukogawa Women's University, Nishinomiya, Hyogo, Japan.
Cryo Letters. 2011 May-Jun;32(3):206-15.
Cysts of Artemia franciscana are known to be extremely tolerant to UV and ionizing radiation, hypoxia, dryness, osmotic pressure, and temperatures. However, when cysts are hydrated, their resistance to extreme environmental conditions is markedly reduced, and they subsequently enter a developmental sequence. The hatching rate of hydrated cysts declined when they were rapidly frozen after a short period of hydration but slow freezing improved hatching rates after 6-h hydration (1.4 g H2O per g dry wt). We observed that trehalose content in hydrated cysts was greatly reduced up to 6-h time. DSC analysis showed different thermal profiles at two cooling rates, suggesting the formation of a minuscule ice crystal inside the cells. High hatching rates can be obtained from highly hydrated cysts at slow cooling rate.
已知卤虫囊肿对紫外线、电离辐射、缺氧、干燥、渗透压和温度具有极强的耐受性。然而，当囊肿水化时，它们对极端环境条件的抵抗力会显著降低，随后进入发育序列。水化囊肿在短时间水化后快速冷冻时孵化率下降，但在水化6小时（每克干重1.4克水）后缓慢冷冻可提高孵化率。我们观察到，水化囊肿中的海藻糖含量在6小时内大幅降低。差示扫描量热法分析显示，在两种冷却速率下有不同的热曲线，表明细胞内形成了微小的冰晶。在缓慢冷却速率下，高度水化的囊肿可获得高孵化率。
