Reproductive Science Group, School of Environmental and Life Sciences, University of Newcastle, Callaghan, New South Wales 2308, Australia.
IUBMB Life. 2011 Sep;63(9):678-85. doi: 10.1002/iub.499. Epub 2011 Jul 15.
Mammalian gametogenesis is a complex process involving specialised cell cycle progression and differentiation. As part of their differentiation, germ cells experience periods of transcriptional inactivation and chromatin inaccessibility whilst continuing to coordinate the correct temporal and spatial expression of genes required for continued development. To overcome these obstacles, mammalian germ cells express a wide variety of sequence-specific RNA-binding proteins, which assist in the translational control of many mRNA transcripts which are produced and stored during periods of high mRNA synthesis. In this review we focus on the Musashi family of RNA-binding proteins, a highly conserved family of translational regulatory proteins whose recent identification in germ cells of Drosophila and Xenopus, as well as their well described role in processes such as cell cycle progression and stem cell identity, has led us to investigate the role of these proteins in mammalian germ cell development.
哺乳动物配子发生是一个复杂的过程，涉及专门的细胞周期进程和分化。作为其分化的一部分，生殖细胞经历转录失活和染色质不可及的时期，同时继续协调继续发育所需的基因的正确时空表达。为了克服这些障碍，哺乳动物生殖细胞表达广泛的序列特异性 RNA 结合蛋白，这些蛋白有助于翻译控制许多 mRNA 转录本的表达，这些转录本在高 mRNA 合成期间产生和储存。在这篇综述中，我们专注于 Musashi 家族的 RNA 结合蛋白，这是一个高度保守的翻译调节蛋白家族，其在果蝇和爪蟾生殖细胞中的最近鉴定，以及它们在细胞周期进程和干细胞特性等过程中的明确作用，促使我们研究这些蛋白在哺乳动物生殖细胞发育中的作用。
