Ottawa-Carleton Chemistry Institute, Department of Chemistry, Carleton University, Ottawa, ON, Canada.
Electrophoresis. 2011 Aug;32(16):2181-7. doi: 10.1002/elps.201100106. Epub 2011 Jul 18.
Hazardous compounds and bacteria in water have an adverse impact on human health and environmental ecology. Polydopamine (or polypyrrole)-coated magnetic nanoparticles and polymethacrylic acid-co-ethylene glycol dimethacrylate submicron particles were investigated for their fast binding kinetics with bisphenol A, proflavine, naphthalene acetic acid, and Escherichia coli. A new method was developed for the rapid determination of % binding by sequential injection of particles first and compounds (or E. coli) next into a fused-silica capillary for overlap binding during electrophoretic migration. Only nanolitre volumes of compounds and particles were sufficient to complete a rapid binding test. After heterogeneous binding, separation of the compounds from the particles was afforded by capillary electrophoresis. % binding was influenced by applied voltage but not current flow. In-capillary coating of particles affected the % binding of compounds.
水中的有害化合物和细菌对人类健康和环境生态有不良影响。本研究考察了聚多巴胺（或聚吡咯）包覆的磁性纳米粒子和聚甲基丙烯酸-co-乙二醇二甲基丙烯酸酯亚微米粒子与双酚 A、吖啶黄素、萘乙酸和大肠杆菌的快速结合动力学。通过顺序注射纳米粒子和化合物（或大肠杆菌）进入熔融石英毛细管，在电泳迁移过程中进行重叠结合，开发了一种快速测定结合百分比的新方法。只需纳升级的化合物和粒子体积即可完成快速结合测试。在异质结合后，通过毛细管电泳将化合物与粒子分离。结合百分比受外加电压影响，但不受电流影响。在管内涂覆粒子会影响化合物的结合百分比。
