Department of Chemistry, Ottawa-Carleton Chemistry Institute, Carleton University, Ottawa, ON, Canada.
Electrophoresis. 2012 Apr;33(8):1255-62. doi: 10.1002/elps.201100706.
Capillary electrophoresis with ultraviolet detection (CE-UV) was used to perform competitive binding tests to demonstrate the selective recognition of bisphenol A (BPA) by molecularly imprinted polymer (MIP) particles. Cross-linking polymerization of methacrylic acid (MAA) and ethylene glycol dimethacrylate (EGDMA) in the presence of BPA yielded MIP particles with an average diameter of 164 ± 15 nm. Their ability to recognize BPA in the presence of nonionic, anionic, and cationic water contaminants was investigated. Binding efficiency was rapidly determined, after sequential injection of particles first and compounds next into the fused-silica capillary provided a short overlapping time during their electrophoretic migrations. The MIP particles exhibited high-binding efficiency (99 ± 1%) for BPA. Neither diclofenac nor metformin affected BPA binding, and 2-hydroxy-4-methoxybenzophenone was even displaced from the particles by BPA. These results verified the high selectivity of MIP toward its target compound.
采用毛细管电泳-紫外检测（CE-UV）技术进行竞争结合试验，以证明分子印迹聚合物（MIP）颗粒对双酚 A（BPA）的选择性识别。在 BPA 的存在下，甲基丙烯酸（MAA）和乙二醇二甲基丙烯酸酯（EGDMA）的交联聚合生成了平均直径为 164 ± 15nm 的 MIP 颗粒。研究了它们在存在非离子、阴离子和阳离子水污染物的情况下对 BPA 的识别能力。通过顺序注射颗粒和化合物首先进入熔融石英毛细管，在它们的电泳迁移过程中提供短的重叠时间，快速确定了结合效率。MIP 颗粒对 BPA 表现出高的结合效率（99 ± 1%）。双氯芬酸和二甲双胍都不会影响 BPA 的结合，甚至 2-羟基-4-甲氧基二苯甲酮也会被 BPA 从颗粒中取代。这些结果验证了 MIP 对其目标化合物的高选择性。
