Department of Analytical Chemistry and Food Technology, University of Castilla-La Mancha, Av. Camilo José Cela 10, E-13004, Ciudad Real, Spain.
Talanta. 2012 Sep 15;99:897-903. doi: 10.1016/j.talanta.2012.07.053. Epub 2012 Jul 25.
A rapid and selective method for the extraction and determination of catecholamines (CLs) from urine samples has been successfully developed using a magnetic molecularly imprinted polymer (MMIP) as a sorbent material. The MMIP has been prepared using dopamine hydrochloride (DA) as template molecule, methacrylic acid (MAA) as functional monomer, ethylene glycol dimethacrylate (EDMA) as cross-linking agent and Fe(3)O(4) magnetite as magnetic component. The extraction was carried out by stirring urine samples with the magnetic polymer. When the extraction was completed, the MMIP, together with the captured analytes, was easily separated from the sample matrix by an adscititious magnet. The analytes desorbed from the MMIP were determined by capillary electrophoresis (CE). It was shown that the MMIP had high affinity and selectivity toward DA and other structurally related CLs such as 3-methoxytyramine hydrochloride (MT), DL-normetanephrine hydrochloride (NME), DL-norephinephrine hydrochloride (NE) and (±) epinephrine (E). Different parameters affecting the extraction efficiency were evaluated in order to achieve the optimal pre-concentration of the analytes and to reduce non-specific interactions. Under the optimal conditions, the CL limits of detection were at the 0.04-0.06 μM range. The relative standard deviations of migration time and response ranged from 0.7% to 1.4% and from 2.9% to 5.5%, respectively. The proposed method was successfully applied to determine CLs, including MT, NME, DA, NE and E in human urine samples.
一种快速、选择性的从尿液样本中提取和测定儿茶酚胺（CLs）的方法已经成功开发，使用磁性分子印迹聚合物（MMIP）作为吸附材料。MMIP 是使用盐酸多巴胺（DA）作为模板分子、甲基丙烯酸（MAA）作为功能单体、乙二醇二甲基丙烯酸酯（EDMA）作为交联剂和 Fe3O4 磁铁矿作为磁性成分制备的。通过搅拌尿液样本与磁性聚合物进行萃取。萃取完成后，MMIP 与捕获的分析物一起，通过外加磁场很容易从样品基质中分离出来。从 MMIP 上解吸的分析物通过毛细管电泳（CE）进行测定。结果表明，MMIP 对 DA 和其他结构相关的 CLs（如 3-甲氧基酪胺盐酸盐（MT）、DL-去甲肾上腺素盐酸盐（NME）、DL-去甲肾上腺素盐酸盐（NE）和（±）肾上腺素（E））具有高亲和力和选择性。为了实现分析物的最佳预浓缩并减少非特异性相互作用，评估了影响萃取效率的不同参数。在最佳条件下，CL 的检测限在 0.04-0.06 μM 范围内。迁移时间和响应的相对标准偏差分别在 0.7%至 1.4%和 2.9%至 5.5%之间。该方法成功应用于测定人尿液样本中的 CLs，包括 MT、NME、DA、NE 和 E。
