嗜热栖热菌HB8中假定插入序列IS1000的克隆与测序
Cloning and sequence of IS1000, a putative insertion sequence from Thermus thermophilus HB8.
作者信息
Ashby M K, Bergquist P L
机构信息
Department of Cellular and Molecular Biology, University of Auckland, New Zealand.
出版信息
Plasmid. 1990 Jul;24(1):1-11. doi: 10.1016/0147-619x(90)90020-d.
The complete nucleotide sequences of two copies of a putative insertion sequence IS1000 from Thermus thermophilus HB8 are presented. IS1000 is 1196 base pairs long, contains a long open reading frame which could code for a protein of 317 amino acids, and has imperfect terminal inverted repeats of 6 base pairs (confirmed by the terminal sequencing of 4.5 copies of IS1000), but does not cause a target site duplication. There are at least 6 copies of IS1000 in the genome of T. thermophilus HB8. A search of the GEN-EMBL data base revealed that the putative 317 amino acid protein had significant homology with open reading frames in the transposable elements IS110 of Streptomyces coelicolor and IS492 of Pseudomonas atlantica.
本文给出了嗜热栖热菌HB8中假定插入序列IS1000的两个拷贝的完整核苷酸序列。IS1000长1196个碱基对，包含一个长的开放阅读框，该阅读框可编码一个由317个氨基酸组成的蛋白质，并且具有6个碱基对的不完全末端反向重复序列（通过对4.5个IS1000拷贝进行末端测序得以证实），但不会导致靶位点重复。嗜热栖热菌HB8的基因组中至少有6个IS1000拷贝。对GEN-EMBL数据库的搜索显示，假定的317个氨基酸的蛋白质与天蓝色链霉菌的转座元件IS110和大西洋假单胞菌的IS492中的开放阅读框具有显著同源性。
Zotero 插件