嗜热细菌嗜火栖热菌染色体的物理图谱。
A physical map of the hyperthermophilic bacterium Aquifex pyrophilus chromosome.
作者信息
Shao Z, Mages W, Schmitt R
机构信息
Lehrstuhl für Genetik, Universität Regensburg, Germany.
出版信息
J Bacteriol. 1994 Nov;176(21):6776-80. doi: 10.1128/jb.176.21.6776-6780.1994.
Abstract
A genomic map of the hyperthermophilic hydrogen-oxidizing bacterium Aquifex pyrophilus was established with NotI (GC/GGCCGC), SpeI (A/CTAGT), and XbaI (T/CTAGA). Linking clones and cross-hybridization of restriction fragments revealed a single circular chromosome of 1.6 Mbp. A single flagellin gene and six rRNA gene units were located on this map by Southern hybridization.
摘要
利用NotI（GC/GGCCGC）、SpeI（A/CTAGT）和XbaI（T/CTAGA）构建了嗜热氢氧化细菌嗜热栖热菌的基因组图谱。连接克隆和限制片段的交叉杂交显示有一条1.6 Mbp的单环染色体。通过Southern杂交在该图谱上定位了一个鞭毛蛋白基因和六个rRNA基因单元。
Zotero 插件