Instituto de Investigaciones Marinas, CSIC, Vigo, Spain.
J Appl Microbiol. 2011 Oct;111(4):800-10. doi: 10.1111/j.1365-2672.2011.05104.x. Epub 2011 Aug 4.
Investigation of the alkane-degrading properties of Dietzia sp. H0B, one of the isolated Corynebacterineae strains that became dominant after the Prestige oil spill.
Using molecular and chemical analyses, the alkane-degrading properties of strain Dietzia sp. H0B were analysed. This Grampositive isolate was able to grow on n-alkanes ranging from C₁₂ to C₃₈ and branched alkanes (pristane and phytane). 8-Hexadecene was detected as an intermediate of hexadecane degradation by Dietzia H0B, suggesting a novel alkane-degrading pathway in this strain. Three putative alkane hydroxylase genes (one alkB homologue and two CYP153 gene homologues of cytochrome P450 family) were PCR-amplified from Dietzia H0B and differed from previously known hydroxylase genes, which might be related to the novel degrading activity observed on Dietzia H0B. The alkane degradation activity and the alkB and CYP153 gene expression were observed constitutively regardless of the presence of the substrate, suggesting additional, novel pathways for alkane degradation.
The results from this study suggest novel alkane-degrading pathways in Dietzia H0B and a genetic background coding for two different putative oil-degrading enzymes, which is mostly unexplored and worth to be subject of further functional analysis.
This study increases the scarce information available about the genetic background of alkane degradation in genus Dietzia and suggests new pathways and novel expression mechanisms of alkane degradation.
研究在“威望”号石油泄漏事件后占优势地位的棒杆菌属菌株之一 Dietzia sp. H0B 的烷烃降解特性。
使用分子和化学分析，分析了菌株 Dietzia sp. H0B 的烷烃降解特性。该革兰氏阳性菌能够在 C₁₂ 到 C₃₈ 的正烷烃和支链烷烃（姥鲛烷和植烷）上生长。通过 Dietzia H0B 检测到十六烷降解的中间产物 8-十六烯，表明该菌株存在一种新的烷烃降解途径。从 Dietzia H0B 中 PCR 扩增了三个假定的烷烃羟化酶基因（一个 alkB 同源物和两个细胞色素 P450 家族的 CYP153 基因同源物），与先前已知的羟化酶基因不同，这可能与在 Dietzia H0B 上观察到的新降解活性有关。无论底物是否存在，烷烃降解活性以及 alkB 和 CYP153 基因的表达均呈组成型，表明存在用于烷烃降解的其他新途径。
该研究结果表明 Dietzia H0B 中存在新的烷烃降解途径和编码两种不同假定的石油降解酶的遗传背景，这在很大程度上尚未得到探索，值得进一步进行功能分析。
本研究增加了关于 Dietzia 属中烷烃降解遗传背景的稀缺信息，并提出了新的烷烃降解途径和新的表达机制。
